PMID: 11337046May 5, 2001Paper

Detection of arboviral RNA directly from mosquito homogenates by reverse-transcription-polymerase chain reaction

Journal of Virological Methods
C HuangD J White

Abstract

Many arthropod-borne viruses (arboviruses) are important human pathogens medically. The development of an effective technique to detect the viruses by using nucleic acid amplification, such as polymerase chain reaction (PCR), improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. In this study, the development of an improved and simplified assay is described for detection of mosquitoes infected with eastern equine encephalitis (EEE) virus, Cache Valley (CV), and California (CAL) serogroup viruses from field-collected mosquito pools. As little as 5 microl of homogenate from mosquito pools was used in the reverse transcription (RT) reaction followed by the use of three sets of specific primers for the PCR. Positive pools were determined by finding PCR bands of the expected size for each arbovirus. The confirmation and identification of Bunyaviruses was done by sequencing the PCR product. In 1999, West Nile virus (WNV) was identified as the etiologic agent of an outbreak of human encephalitis in New York City. It is shown that this protocol is also able to detect West Nile viral RNA in a pool of 100 mosquitoes containing one infected mosquito.

References

Jan 1, 1992·Journal of Virological Methods·D K HoweG L McLaughlin
Apr 5, 1996·Journal of Virological Methods·W P Campbell, C Huang

❮ Previous
Next ❯

Citations

May 6, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·A P G AlmeidaA Esteves
Sep 10, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cinnia HuangLeo Grady
Jan 19, 2006·Vector Borne and Zoonotic Diseases·Aida EstevesM Teresa Novo
Sep 22, 2010·Vector Borne and Zoonotic Diseases·António Paulo G AlmeidaAida Esteves
Oct 19, 2004·Clinical Microbiology Reviews·Roberta L Debiasi, Kenneth L Tyler
Jan 9, 2003·Journal of Clinical Microbiology·Amy J LambertRobert S Lanciotti
Aug 9, 2003·Journal of Clinical Microbiology·Elizabeth B KauffmanLaura D Kramer
Apr 9, 2005·Journal of Clinical Microbiology·Amy J LambertRobert S Lanciotti
Jul 14, 2010·Virology Journal·Mario L G de FigueiredoLuiz T M Figueiredo
Apr 30, 2014·Journal of the American Mosquito Control Association·Leah P MayJames E Maruniak
Jan 23, 2008·Enfermedades infecciosas y microbiología clínica·Viviana RéMarta Contigiani
Oct 5, 2006·Journal of Medical Entomology·Kiet A NgoLaura D Kramer
Nov 25, 2003·Expert Review of Molecular Diagnostics·Pei-Yong Shi, Susan J Wong
Jan 11, 2017·Journal of Medical Entomology·P Kate CarsonThomas W Chapman
Apr 16, 2021·Biomedical Microdevices·Viviane Klassen de OliveiraLucas Blanes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.