Detection of Arcobacter species in gastric samples from swine

Veterinary Microbiology
D L SuarezD J Larson

Abstract

Swine stomachs were surveyed for evidence of Arcobacter spp. and Helicobacter spp. infections associated with gastric ulceration. A nested PCR test targeted to the 16S rRNA was developed to detect many Arcobacter spp. and Helicobacter spp. An internal oligonucleotide probe was used for differentiation and confirmation of the PCR product. Tissue samples were obtained from the nonglandular and glandular regions of 86 swine stomachs. Evidence of infection with these microbes was detected in 51%, with 77% of the positive samples being identified as A. butzleri using a highly specific probe. Nonglandular stomach samples (44%) were more likely to be positive by PCR than samples from the glandular (23%) region. Gross lesions of any stage of gastric ulceration, ranging from parakeratosis, erosions and ulceration, were observed in 24% of stomachs examined. Of 21 samples with lesions, 52% were positive by the broadly reactive PCR assay for Arcobacter spp. and Helicobacter spp. The majority of PCR-positive samples (75%) had no gross lesions. When a single step PCR assay that was more specific for Arcobacter spp. was used on the nonglandular stomach samples, 10.4% of the 86 samples were positive. Arcobacter spp. were cultured from four of ...Continue Reading

References

Aug 1, 1990·Veterinary Microbiology·D M QueirozA J Barbosa
Jul 1, 1994·International Journal of Systematic Bacteriology·F E DewhirstJ G Fox
Apr 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·L Schroeder-TuckerG A Erickson

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Citations

Apr 9, 2002·Preventive Veterinary Medicine·I D RobertsonD J Hampson
Feb 18, 2004·International Journal of Food Microbiology·Lucia RivasPaul Vanderlinde
Dec 19, 2003·FEMS Microbiology Letters·Ellen van DriesschePeter Vandamme
Mar 12, 2013·International Journal of Food Microbiology·K A ScanlonG Duffy
Jun 26, 2007·International Journal of Food Microbiology·Ellen Van Driessche, Kurt Houf
Nov 14, 2006·Journal of Microbiological Methods·Kurt Houf, Roger Stephan
Feb 27, 2008·Journal of Applied Microbiology·E Van Driessche, K Houf
Jul 25, 1998·The Journal of Biological Chemistry·N ZakharovaK Severinov
Mar 19, 2005·International Journal of Systematic and Evolutionary Microbiology·Kurt HoufPeter Vandamme
May 12, 2001·Journal of Food Protection·M E HumeH B Zhang
May 13, 2008·Journal of Food Protection·M PejchalováJ Vytrasová
Oct 3, 1999·Applied and Environmental Microbiology·F CantetF Mégraud

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