PMID: 2101521Jul 1, 1990Paper

Detection of asymptomatic carriers of hydatid cysts: specificity increase of the immunoenzyme assay

Revista Do Instituto De Medicina Tropical De São Paulo
E A ColtortiE A Guarnera

Abstract

An enzymoimmunoassay (EIE) as a screening test to select potential asymptomatic cyst carriers among the general population of areas under risk is being used in programs for the control of hydatic diseases caused by Echinococcus granulosus in Argentina. The experience obtained up to date, applying this assay in population surveys, indicates that depending on the prevalence in the area 10% to 30% of the individuals selected did not show images compatible with hydatic cysts. The purpose of the present study was to improve the specificity of the test. To this purpose, the influence of the modification of the antigenic availability and the effect of the absorption from the serum samples of antibodies anti-normal ovine sera and anti-phosphorylcholine was evaluated. One hundred and fourteen non hydatic sera selected because of their high cross reactivity in EIE using the whole hydatid antigen (WHA) and 118 hydatid sera, were studied with four fractions of ovine hydatid cyst fluid. The EIE employing the S2B antigenic fraction with previous absorption of the sera (EIE-S2B/A) was the system that discriminated better hydatid sera from non hydatid sera with high levels of cross reactivity. The replacement of the EIE employing WHA by the EI...Continue Reading

References

Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·E A Coltorti, V M Varela-Díaz
Sep 1, 1987·Molecular and Biochemical Parasitology·J C Shepherd, D P McManus
Apr 1, 1989·The American Journal of Tropical Medicine and Hygiene·S E MaddisonV C Tsang
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·E ColtortiA Aquino
May 1, 1988·The American Journal of Tropical Medicine and Hygiene·E ColtortiJ Iriarte
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Jul 1, 1971·The American Journal of Tropical Medicine and Hygiene·R OriolC Oriol

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Citations

Mar 1, 1995·Revista Do Instituto De Medicina Tropical De São Paulo·G M Leal-BacelarL C da Silva
Mar 1, 1993·Revista Do Instituto De Medicina Tropical De São Paulo·E Coltorti, G Cammarieŕi
Sep 19, 2020·Experimental Parasitology·Maria Florencia SierraI E Sommerfelt

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