Detection of asymptomatic renal Leptospira infection in abattoir slaughtered cattle in southeastern Georgia, United States

SAGE Open Medicine
Sreekumari RajeevMel E Pence

Abstract

Leptospirosis is one of the most widespread zoonotic infectious diseases affecting humans and animals. Several animal species, including cattle, can act as potential asymptomatic carriers facilitating zoonotic transmission of Leptospira. This study was conducted to assess the occurrence of asymptomatic renal Leptospira carriers among cattle slaughtered in southeastern Georgia, United States. A battery of diagnostic tests, including dark field microscopy, direct fluorescent antibody staining, polymerase chain reaction, and culture, were performed on a set of bovine kidneys (n = 37) collected from an abattoir in southeastern Georgia, United States. Virulence of a field isolate obtained from this study was tested in a hamster experimental model. Motile spirochete-like structures were observed by dark field microscopy in 23 (59%) out of 37 kidney samples tested. In all, 29 samples (78%) were positive by direct fluorescent antibody staining. Only 11 (29.7%) samples by polymerase chain reaction and 3 (8.1%) by culture were positive for Leptospira sp. The isolates obtained by culture were confirmed as Leptospira borgpetersenii. Hamsters experimentally infected with one of the Leptospira field isolates obtained from this study did not ...Continue Reading

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Citations

Sep 16, 2016·Tropical Animal Health and Production·Vahideh TaghadosiAzadeh Samiei
Jun 29, 2018·International Journal of Microbiology·Alejandro LlanesSreekumari Rajeev
Feb 27, 2020·Animals : an Open Access Journal From MDPI·Sreekumari RajeevEsteban Ellis
Oct 19, 2020·The American Journal of Tropical Medicine and Hygiene·Christine AtherstoneSiobhan M Mor
Apr 25, 2018·Veterinary Microbiology·Jarlath E NallyNathan E Bauer

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Methods Mentioned

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