Detection of autoantibodies against aquaporin-5 in the sera of patients with primary Sjögren's syndrome

Immunologic Research
Jehan AlamYoungnim Choi

Abstract

The pathophysiology of exocrine dysfunction observed in Sjögren's syndrome (SS) is not fully understood. The purpose of this study was to investigate whether autoantibodies against human AQP5 are present in the sera of SS patients. Frozen sections of mouse submandibular salivary glands, CHO cells over-expressing a human AQP5-GFP fusion protein or GFP, and MDCK cells over-expressing AQP5 were used in the indirect immunofluorescence assay to detect anti-AQP5 autoantibodies in the sera from patients with primary SS. The lysates of HEK-293 cells over-expressing the AQP5-GFP fusion protein or GFP were used for immunoprecipitation. Serum IgG from the SS patients but not from the control subjects stained acinar cells in the mouse salivary glands, the signals of which colocalized with those of AQP5-specific antibodies. Serum IgG from the SS patients also selectively stained AQP5-GFP expressed in CHO cells. However, both the control and SS sera immunoprecipitated the AQP5-GFP, suggesting that autoantibodies against AQP5 were also present in the control sera. The screening of 53 control and 112 SS samples by indirect immunofluorescence assay using the AQP5-expressing MDCK cells revealed the presence of significantly higher levels of anti...Continue Reading

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Citations

Jul 1, 2017·Journal of Dental Research·J AlamY Choi
Oct 3, 2017·Rheumatology·John S TzartosSocrates J Tzartos
Nov 2, 2018·International Journal of Molecular Sciences·Muhammad Shahnawaz SoyfooChristine Delporte
Jul 8, 2020·Cells·Claudia D'AgostinoChristine Delporte
May 22, 2020·Inflammation·Lezy Flora Mariajoseph-AntonyChidambaram Prahalathan
Apr 13, 2021·Journal of Dental Research·S M LeeD Y Kim
Jul 31, 2021·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Reza Rastmanesh

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Methods Mentioned

BETA
transfection
confocal microscopy
immunoprecipitation

Software Mentioned

SPSS

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