PMID: 7543794Apr 1, 1995Paper

Detection of autoantibodies against bullous pemphigoid and pemphigus antigens by an enzyme-linked immunosorbent assay using the bacterial recombinant proteins

Experimental Dermatology
A IdeT Nishikawa

Abstract

To develop a new method to evaluate autoantibodies in various autoimmune bullous skin diseases, we examined reactivity of bullous pemphigoid (BP) and pemphigus vulgaris (PV) patients' sera with partial bacterial fusion proteins of the 230 kD BP antigen (BPAG1) and PV antigen, respectively, by an enzyme-linked immunosorbent assay (ELISA), and compared the results with those of immunoblotting. We used two fusion proteins derived from the mouse BPAG1 and a fusion protein derived from the amino-terminus (EC1-2) of PV antigen. For both BP and PV sera, the ELISA scores were well correlated with the reactivities on immunoblot assays. The present study indicates that ELISA using recombinant antigen proteins in various autoimmune bullous skin diseases will be a new useful technique to detect the autoantibodies. However, development of recombinant proteins with the entire molecule and correct conformation will be necessary to establish a perfect ELISA system in the future.

References

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Citations

Dec 21, 2007·Clinical Reviews in Allergy & Immunology·Michael Kasperkiewicz, Detlef Zillikens
Apr 20, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Arno KrommingaDetlef Zillikens
Jan 13, 2001·Journal of Dermatological Science·Y OhataT Hashimoto
May 28, 2003·Protein Expression and Purification·Emmanuel LaffitteLuca Borradori
May 9, 2012·Immunology and Allergy Clinics of North America·Enno SchmidtLuca Borradori
May 25, 2011·Dermatologic Clinics·Enno SchmidtLuca Borradori
Dec 15, 2010·Clinics in Dermatology·Sareeta R S Parker, Jamie MacKelfresh
Jun 24, 2008·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Giovanni Di ZenzoMichael Hertl
Dec 28, 2007·Advances in Dermatology·Giovanni Di ZenzoLuca Borradori
Aug 28, 2009·Journal of the European Academy of Dermatology and Venereology : JEADV·Z HallajiC Chams-Davatchi
Apr 27, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Otto Van de GaerXavier Bossuyt

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