Detection of autoantibodies against thyroid peroxidase in serum samples of hypothyroid dogs

American Journal of Veterinary Research
Evelyn SkopekR Nachreiner

Abstract

To establish a sensitive test for the detection of autoantibodies against thyroid peroxidase (TPO) in canine serum samples. 365 serum samples from dogs with hypothyroidism as determined on the basis of serum concentrations of total and free triiodothyronine (T3), total and free thyroxine (T4), and thyroid-stimulating hormone, of which 195 (53%) had positive results for at least 1 of 3 thyroid autoantibodies (against thyroglobulin [Tg], T4, or T3) and serum samples from 28 healthy dogs (control samples). TPO was purified from canine thyroid glands by extraction with detergents, ultracentrifugation, and precipitation with ammonium sulfate. Screening for anti-TPO autoantibodies in canine sera was performed by use of an immunoblot assay. Thyroid extract containing TPO was separated electrophoretically, blotted, and probed with canine sera. Alkaline phosphatase-conjugated rabbit anti-dog IgG was used for detection of bound antibodies. TPO bands were observed at 110, 100, and 40 kd. Anti-TPO autoantibodies against the 40-kd fragment were detected in 33 (17%) sera of dogs with positive results for anti-Tg, anti-T4, or anti-T3 autoantibodies but not in sera of hypothyroid dogs without these autoantibodies or in sera of healthy dogs. Th...Continue Reading

References

Jun 1, 1977·Endocrinology·N M Alexander
Feb 1, 1979·The Journal of Clinical Endocrinology and Metabolism·S MariottiL Baschieri
Feb 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·T Nakashima, A Taurog
Feb 1, 1990·The Journal of Antimicrobial Chemotherapy·R R Coombs
Feb 1, 1990·Clinical and Experimental Immunology·S M McLachlanB Rees Smith
May 1, 1986·The Journal of Clinical Endocrinology and Metabolism·T KotaniS Ohtaki
Jul 1, 1970·Archives of Biochemistry and Biophysics·A TaurogR W Estabrook
Apr 14, 1971·Biochimica Et Biophysica Acta·D J Danner, M Morrison
Oct 1, 1984·The Journal of Clinical Endocrinology and Metabolism·U BognerJ R Wall
Jun 14, 1984·Biochimica Et Biophysica Acta·J M Van Zyl, B J Van der Walt
Jan 1, 1997·Journal of Nurse-midwifery·L VandeVusse
May 26, 1999·The Journal of Small Animal Practice·D L Panciera
Mar 27, 2002·Nature Reviews. Immunology·Giorgio Stassi, Ruggero De Maria
Apr 2, 2003·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·M Patzl, E Möstl

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Citations

Jul 10, 2007·The Veterinary Clinics of North America. Small Animal Practice·Peter A GrahamRaymond F Nachreiner
Jul 10, 2007·The Veterinary Clinics of North America. Small Animal Practice·Duncan C Ferguson
Dec 30, 2014·Biochimica Et Biophysica Acta·Takanori MoriyamaNaoyuki Honma
Jun 23, 2021·Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere·Sina StreyJohanna Rieder

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Autoimmune thyroiditis is an inflammatory disease of thyroid gland due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-cells and thyroid autoantibodies. Discover the latest research on autoimmune thyroiditis here.

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