Abstract
To establish a sensitive test for the detection of autoantibodies against thyroid peroxidase (TPO) in canine serum samples. 365 serum samples from dogs with hypothyroidism as determined on the basis of serum concentrations of total and free triiodothyronine (T3), total and free thyroxine (T4), and thyroid-stimulating hormone, of which 195 (53%) had positive results for at least 1 of 3 thyroid autoantibodies (against thyroglobulin [Tg], T4, or T3) and serum samples from 28 healthy dogs (control samples). TPO was purified from canine thyroid glands by extraction with detergents, ultracentrifugation, and precipitation with ammonium sulfate. Screening for anti-TPO autoantibodies in canine sera was performed by use of an immunoblot assay. Thyroid extract containing TPO was separated electrophoretically, blotted, and probed with canine sera. Alkaline phosphatase-conjugated rabbit anti-dog IgG was used for detection of bound antibodies. TPO bands were observed at 110, 100, and 40 kd. Anti-TPO autoantibodies against the 40-kd fragment were detected in 33 (17%) sera of dogs with positive results for anti-Tg, anti-T4, or anti-T3 autoantibodies but not in sera of hypothyroid dogs without these autoantibodies or in sera of healthy dogs. Th...Continue Reading
References
Jun 1, 1977·Endocrinology·N M Alexander
Feb 1, 1979·The Journal of Clinical Endocrinology and Metabolism·S MariottiL Baschieri
Feb 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·T Nakashima, A Taurog
Apr 1, 1990·Clinical and Experimental Immunology·T KotaniS Ohtaki
Feb 1, 1990·The Journal of Antimicrobial Chemotherapy·R R Coombs
Feb 1, 1990·Clinical and Experimental Immunology·S M McLachlanB Rees Smith
May 1, 1990·Archives of Biochemistry and Biophysics·A TaurogC Slaughter
Sep 1, 1988·Molecular Endocrinology·N Yokoyama, A Taurog
May 1, 1986·The Journal of Clinical Endocrinology and Metabolism·T KotaniS Ohtaki
Jul 1, 1970·Archives of Biochemistry and Biophysics·A TaurogR W Estabrook
Apr 14, 1971·Biochimica Et Biophysica Acta·D J Danner, M Morrison
Oct 1, 1984·The Journal of Clinical Endocrinology and Metabolism·U BognerJ R Wall
Jun 14, 1984·Biochimica Et Biophysica Acta·J M Van Zyl, B J Van der Walt
Jan 1, 1997·Journal of Nurse-midwifery·L VandeVusse
May 26, 1999·The Journal of Small Animal Practice·D L Panciera
Jun 8, 1999·European Journal of Immunology·C DuthoitJ Ruf
Mar 27, 2002·Nature Reviews. Immunology·Giorgio Stassi, Ruggero De Maria
Apr 2, 2003·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·M Patzl, E Möstl
Citations
Dec 5, 2012·ISRN Neurology·R AlroughaniS F Ahmed
Jul 10, 2007·The Veterinary Clinics of North America. Small Animal Practice·Peter A GrahamRaymond F Nachreiner
Jul 10, 2007·The Veterinary Clinics of North America. Small Animal Practice·Duncan C Ferguson
Mar 10, 2010·Tissue Antigens·M WilbeS Björnerfeldt
Dec 30, 2014·Biochimica Et Biophysica Acta·Takanori MoriyamaNaoyuki Honma
Dec 1, 2015·PloS One·Alisdair M BoagBrian Catchpole
Jan 23, 2019·Journal of Veterinary Internal Medicine·Julia D LabadieAnne C Avery
Aug 14, 2010·Journal of Veterinary Science·Marion PiechottaHans Otto Hoppen
Jun 1, 2021·Veterinary Pathology·Jana JankovicMiguel Campos
Jun 23, 2021·Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere·Sina StreyJohanna Rieder