PMID: 2096010Dec 1, 1990Paper

Detection of autoantibodies to recombinant human thyroid peroxidase by sensitive enzyme immunoassay

Clinical Endocrinology
D L KendlerT F Davies

Abstract

Autoantibodies to thyroid peroxidase (TPO), the thyroid 'microsomal' antigen, are widely utilized in the diagnosis of human autoimmune thyroid disease. Crude human thyroid preparations of TPO are of differing potency, contain residual thyroglobulin (Tg) and other human membrane antigens, and are available in only limited amounts. Hence, immunoassays for anti-TPO are unstandardized and of variable sensitivity and specificity. We co-transfected the Chinese hamster ovary (CHO) cell line with a full-length human TPO cDNA expression plasmid. We selected a high expressing recombinant TPO positive cell population (CHO-TPO) by Northern blot analysis, then fluorescence laser flow cytometry using both human polyclonal and murine monoclonal anti-TPO antibodies. Solubilized 100,000 g membrane preparations from both CHO-TPO and CHO cells were used as antigens in a specific ELISA with CHO antigen serving as background control. In a selected series of known anti-TPO positive (n = 46) and negative (n = 73) sera there was a high correlation between ELISAs utilizing recombinant or natural-TPO antigen (r = 0.93). There appeared to be no difference in the affinity of high titre human anti-TPO for recombinant and natural-TPO antigen with both ELISA...Continue Reading

References

Jan 1, 1987·Annual Review of Immunology·A M Livingstone, C G Fathman
Jul 3, 1989·FEBS Letters·S KimuraT Aoyama
Sep 1, 1989·The Journal of Clinical Endocrinology and Metabolism·R S BahnT J Smith
Nov 1, 1985·The Journal of Clinical Endocrinology and Metabolism·L PortmannL J DeGroot
Nov 1, 1987·Molecular Endocrinology·R P MagnussonB Rapoport
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S KimuraS Ohtaki
Nov 1, 1987·The Journal of Clinical Endocrinology and Metabolism·S MariottiA Pinchera
Jan 1, 1984·Endocrine Reviews·A P Weetman, A M McGregor
Mar 1, 1983·The Journal of Clinical Endocrinology and Metabolism·S MariottiA Pinchera

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Thyroiditis

Autoimmune thyroiditis is an inflammatory disease of thyroid gland due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-cells and thyroid autoantibodies. Discover the latest research on autoimmune thyroiditis here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.