Detection of Avian Influenza Virus from Cloacal Swabs Using a Disposable Well Gate FET Sensor

Advanced Healthcare Materials
Sungwook ParkKwan Hyi Lee

Abstract

Current methods to detect avian influenza viruses (AIV) are time consuming and lo inw sensitivity, necessitating a faster and more sensitive sensor for on-site epidemic detection in poultry farms and urban population centers. This study reports a field effect transistor (FET) based AIV sensor that detects nucleoproteins (NP) within 30 minutes, down to an LOD of 10(3) EID50 mL(-1) from a live animal cloacal swab. Previously reported FET sensors for AIV detection have not targeted NPs, an internal protein shared across multiple strains, due to the difficulty of field-effect sensing in a highly ionic lysis buffer. The AIV sensor overcomes the sensitivity limit with an FET-based platform enhanced with a disposable well gate (DWG) that is readily replaceable after each measurement. In a single procedure, the virus-containing sample is immersed in a lysis buffer mixture to expose NPs to the DWG surface. In comparison with commercial AIV rapid kits, the AIV sensor is proved to be highly sensitive, fast, and compact, proving its potential effectiveness as a portable biosensor.

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Citations

Sep 9, 2017·Advanced Healthcare Materials·Jaebin ChoiKwan Hyi Lee
Dec 14, 2018·Micromachines·Taek LeeMin-Ho Lee
Apr 20, 2021·Biomedical Engineering Letters·Daeun Sung, Jahyun Koo
May 1, 2021·Biosensors·Abbas PanahiEbrahim Ghafar-Zadeh
Jan 8, 2020·ACS Applied Materials & Interfaces·Qin FanXianbao Wang

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