Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry

Biosensors & Bioelectronics
Cai QiGang Jin

Abstract

A novel method is reported for the detection of avian influenza virus subtype H5 using a biosensor based on high spatial resolution imaging ellipsometry (IE). Monoclonal antibodies specific to H5 hemagglutinin protein were immobilized on silicon wafers and used to capture virus particles. Resultant changes on the surface of the wafers were visualized directly in gray-scale on an imaging ellipsometry image. This preliminary study has shown that the assay is rapid and specific for the identification of avian influenza virus subtype H5. Compared with lateral-flow immunoassays, this biosensor not only has better sensitivity, but can also simultaneously perform multiplexed tests. These results suggest that this biosensor might be a valuable diagnostic tool for avian influenza virus detection.

References

Sep 27, 2005·Journal of Virological Methods·Sunchai PayungpornYong Poovorawan
Oct 21, 2006·Electrophoresis·Zhan-Hui WangGang Jin

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Citations

Oct 1, 2010·Annals of Biomedical Engineering·Chenghong HuangGang Jin
Dec 15, 2012·Biosensors & Bioelectronics·Yongbai YinDavid R McKenzie
Sep 24, 2014·Molecular Biology Reports·Taha Roodbar ShojaeiDirk Bald
Oct 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jian'an HeDayong Gu
May 28, 2011·Journal of Colloid and Interface Science·Meng-Jie ChangHao-Li Zhang

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