Detection of avian leukosis virus subgroup J in chicken flocks from Malaysia and their molecular characterization

Avian Pathology : Journal of the W.V.P.A
B R ThapaM Hair-Bejo

Abstract

Previously we have shown that avian leukosis virus subgroup J (ALV-J) might be present in chicken flocks from Malaysia based on serological study and also on detection of tissue samples with myelocytic infiltration. In this study, the polymerase chain reaction was used to detect ALV-J sequences from archived frozen samples. Out of 21 tissue samples examined, 16 samples were positive for proviral DNA and four samples for ALV-J RNA. However, only nine samples were found positive for myelocytic infiltration. A total of 465 base pairs equivalent to positions 5305 to 5769 of HPRS-103 from each of the viral RNA positive samples were characterized. Sequence analysis indicated that the samples showed high identity (95.9 to 98.2%) and were close to HPRS-103 with identities between 97.4 and 99.3%. This study indicates that ALV-J-specific sequences can be detected by polymerase chain reaction from frozen tissue samples with and without myelocytic infiltration.

References

Apr 1, 1991·The Journal of General Virology·L N PayneM E Thouless
Nov 13, 2002·Avian Pathology : Journal of the W.V.P.A·C-H Wang, Y-W Juan
Dec 19, 2003·Avian Pathology : Journal of the W.V.P.A·Binrui XuMaoxiang Li
Dec 1, 2000·Avian Pathology : Journal of the W.V.P.A·A M Fadly
Apr 1, 1999·Avian Pathology : Journal of the W.V.P.A·S S ArshadL N Payne

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Citations

Jul 20, 2010·Tropical Animal Health and Production·Mostafa RajabzadehAli Mohammadi
Mar 16, 2006·Avian Pathology : Journal of the W.V.P.A·H HataiT Umemura
May 15, 2007·Avian Pathology : Journal of the W.V.P.A·Shuhong Sun, Zhizhong Cui
Jun 8, 2012·Journal of Clinical Microbiology·Sathish GopalKurunchi C Divya
Oct 7, 2014·Journal of Virological Methods·Lih-Chiann WangChing-Ho Wang
Aug 21, 2019·Avian Pathology : Journal of the W.V.P.A·Xiaohui MuAijian Qin

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Datasets Mentioned

BETA
IMC10200

Methods Mentioned

BETA
PCR

Software Mentioned

ClustalX

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