Detection of bacterial contamination in starch and resin-based papermaking chemicals using fluorescence techniques

Journal of Industrial Microbiology & Biotechnology
Liisa NohynekLaura Raaska

Abstract

Rapid fluorescence techniques were evaluated for the detection of bacterial contaminants in papermaking chemicals including starch and the resin-based sizes and starch slurries used in the paper industry. Viable and non-viable bacterial cells were visualised by fluorescent probes and detected by epifluorescence microscopy and flow cytometry. The best discrimination ability was obtained with the fluorescent probes LIVE/DEAD and SYBR Green, based on the staining of cellular nucleic acid, and ChemChrome V3, which demonstrated cellular enzymatic activity. The process samples had to be diluted and filtered before fluorescence staining and analysis because they were viscous and contained solid particles. Fluorescence microscopic counts of bacteria in highly contaminated process samples were similar to plate counts, but flow cytometric enumeration of bacterial cells in process samples yielded 2- to 10-fold lower counts compared with plate counts, depending on the consistency of the sample. The detection limits in flow cytometric analysis and in epifluorescence microscopy were 10(3)-10(6) cells ml(-1) and 10(5)-10(6) cells ml(-1), respectively. Intrinsic bacterial contamination was detectable with fluorescence techniques and highly con...Continue Reading

References

Aug 1, 1991·The Journal of Applied Bacteriology·O M VäisänenM S Salkinoja-Salonen
Oct 1, 1986·Applied and Environmental Microbiology·C W Donnelly, G J Baigent
Jan 1, 1993·Biology of the Cell·C Laplace-BuilhéJ L Drocourt
Mar 2, 1999·Applied and Environmental Microbiology·J L TholozanM Federighi
Mar 4, 2000·Applied and Environmental Microbiology·T S GunasekeraD A Veal
May 3, 2000·International Journal of Food Microbiology·P Breeuwer, T Abee
Jun 30, 2000·Methods : a Companion to Methods in Enzymology·M K Winson, H M Davey
Apr 25, 2001·Applied and Environmental Microbiology·C J BunthofT Abee
Jun 23, 2001·Journal of Industrial Microbiology & Biotechnology·T S PirttijärviM S Salkinoja-Salonen
May 3, 2002·Journal of Industrial Microbiology & Biotechnology·A-M SjöbergL Raaska
May 3, 2002·Journal of Industrial Microbiology & Biotechnology·L RaaskaM-L Suihko
May 24, 2003·Journal of Industrial Microbiology & Biotechnology·Johanna MaukonenMaria Saarela
Jul 1, 1995·Applied and Environmental Microbiology·J PorterR Pickup
Sep 1, 1995·Applied and Environmental Microbiology·T TsujiS Tanaka

Citations

Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker

Related Concepts

Equipment Contamination
Flow Cytometry
Fluorochromes
Industrial Microbiology
Immunofluorescence Microscopy
Paper
Keoflo
Streak Plate Count
Resin Cements

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