Detection of bacterial DNA in atheromatous plaques by quantitative PCR

Microbes and Infection
E KozarovDennis Lopatin

Abstract

This is the first study to analyze atheromatous plaques for the presence of bacterial DNA from ten species, including periodontal species and Chlamydia pneumoniae. We examined 129 samples of DNA extracted from atheromas from 29 individuals for the presence of bacterial 16S rDNA sequences from ten different species: Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans (A.a.), Tannerella forsythensis, Eikenella corrodens, Prevotella intermedia, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Treponema denticola and C. pneumoniae. All determinations were made using real-time quantitative polymerase chain reaction (PCR) methods employing SYBR Green. Species from the Bacteroides family were found in about 17% of the young but approximately 80% in elderly patients. Almost half of the samples contained DNA from A. a. and C. pneumoniae, although the proportion of the latter was minimal. S. aureus and S. epidermidis were found with the lowest frequency, 5 and 10%, respectively. S. mutans was found in approximately 20% of the samples. The proportions of each bacterial species were calculated relative to the total amount of prokaryotic DNA. The data support our previous findings of an association between...Continue Reading

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Citations

May 22, 2013·Archives of Microbiology·Soon-Nang ParkJoong-Ki Kook
Jan 21, 2012·Journal of Dental Research·L KesavaluA Progulske-Fox
Mar 23, 2012·Journal of Applied Oral Science : Revista FOB·Janaina Salomon GhizoniAdriana Campos Passanezi Sant'Ana
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