PMID: 2499307Mar 15, 1989Paper

Detection of bacterial lipoic acid. A modified gas-chromatographic-mass-spectrometric procedure

The Biochemical Journal
K J PrattK J Stevenson

Abstract

The detection of bacterial lipoic acid by a modified g.c.-m.s. procedure is reported. Cells were hydrolysed in HCl to release protein-bound lipoic acid, which, after extraction into benzene, was reduced with NaBH4. The dihydrolipic acid so generated was then isolated by covalent chromatography on dithiolspecific p-aminophenylarsenoxide-agarose and, after elution by 2,3-dimercaptopropane-1-sulphonic acid and extraction into benzene, was allowed to O2-oxidize to the disulphide form. The isolated lipoic acid was allowed to react with diazomethane, and the methyl ester so produced was detected by g.c.-m.s. Analysis of the mass spectrum showed the characteristic molecular ion and seven fragmentation ions, which, along with the identification of those ions retaining the two sulphur atoms, allows the definitive detection of lipoic acid. The methodology has been successfully tested with authentic lipoic acid, the 2-oxoglutarate dehydrogenase multienzyme complex and with whole cells of Escherichia coli. In addition, it has been used to search for and identify lipoic acid in the archaebacterium Halobacterium halobium. The significance of this discovery and the possible roles of the cofactor in H. halobium are discussed.

Citations

Dec 12, 2007·PLoS Pathogens·Svenja GüntherSylke Müller
Jan 10, 2017·PloS One·Yirong SunHaihong Wang
Sep 21, 2007·Journal of Pharmaceutical and Biomedical Analysis·Arjumand I DurraniGerhard Sontag
Jun 16, 2007·Extremophiles : Life Under Extreme Conditions·Dina M Al-MailemMichael J Danson
Jul 20, 2011·The Journal of Biological Chemistry·Quin H ChristensenJohn E Cronan
Jan 7, 2011·The Journal of Biological Chemistry·Bachar H Hassan, John E Cronan
Jan 1, 1992·Progress in Biophysics and Molecular Biology·G Wächtershäuser
Dec 1, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·H Kataoka
May 11, 2002·Journal of Bacteriology·Christian Wanner, Jörg Soppa

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