Apr 15, 2008

Detection of bacterial quorum sensing N-acyl homoserine lactones in clinical samples

Analytical and Bioanalytical Chemistry
Anjali KumariS Daunert

Abstract

Bacteria communicate among themselves using certain chemical signaling molecules. These signaling molecules generally are N-acyl homoserine lactones (AHLs) in Gram-negative bacteria and oligopeptides in Gram-positive bacteria. In addition, both Gram-positive and Gram-negative bacteria produce a family of signaling molecules known as autoinducer-2 that they employ for their communications. Bacteria coordinate their behavior by releasing and responding to the chemical signaling molecules present in proportion to their population density. This phenomenon is known as quorum sensing. The role of bacteria in the pathogenesis of several diseases, including gastrointestinal (GI) disorders, is well established. Moreover, rather recently bacterial quorum sensing has been implicated in the onset of bacterial pathogenicity. Thus, we hypothesized that the signaling molecules involved in bacterial communication may serve as potential biomarkers for the diagnosis and management of several bacteria-related diseases. For that, we previously developed a method based on genetically engineered whole-cell sensing systems for the rapid, sensitive, cost-effective and quantitative detection of AHLs in biological samples, such as saliva and stool, from...Continue Reading

Mentioned in this Paper

Gram-Positive Bacteria
Pathogenic Aspects
L-isomer of Homoserine
Pathogenesis
Pathogenicity
Biosensors
Anterior Horizontal Limb of Lateral Sulcus (Human Only)
Saliva - SpecimenType
Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Gastrointestinal Diseases

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