PMID: 6108969Dec 1, 1980Paper

Detection of Bacteroides fragilis and Bacteroides melaninogenicus by direct immunofluorescence

Journal of Clinical Pathology
M LabbéE Yourassowsky

Abstract

A new diagnostic kit, which contains a polyvalent antiserum for either Bacteroides fragilis or Bacteroides melaninogenicus, was tested for reliability and specificity on 146 clinical samples of different origin. A correlation between the culture and immunofluorescence was observed for B. fragilis in 87.39% of cases and for B. melaninogenicus in 81.48% of cases. When pure cultures were tested, aerobically as well as anaerobically, false-positive reactions were observed with staphylococci and Clostridium ramosum spores. The well-defined morphology of these bacteria and spores allows for the elimination of any diagnostic error. The method is rapid, and the margin for error is limited. The test gives a semiquantitative idea of the number of bacteroides organisms present in the clinical specimens even in the presence of a mixed flora.

References

Jan 1, 1977·Journal of Clinical Microbiology·J W HollandW A Altemeier
May 30, 1974·The New England Journal of Medicine·S L Gorbach, J G Bartlett
May 23, 1974·The New England Journal of Medicine·S L Gorbach, J G Bartlett

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Citations

Mar 1, 1989·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·C HöhneG Hübner
May 1, 1988·Journal of Endodontics·E A PanteraM Shih-Levine
Feb 1, 1985·Burns, Including Thermal Injury·D W WangY P Zhan

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