PMID: 8457482Feb 1, 1993Paper

Detection of BCR/ABL transcripts in chronic myeloid leukaemia by polymerase chain reaction and DNA enzyme immunoassay: a DNA probe assay without DNA labelling

British Journal of Haematology
A R MagaliniG Mantero

Abstract

The detection of the t(9;22) translocation, typical of chronic myeloid leukaemia (CML), can be accomplished by cytogenetical detection of Philadelphia (Ph1) chromosome or by molecular analysis of the bcr/abl fusion gene with nucleic acid probes after amplification by polymerase chain reaction (PCR). PCR-based approaches are now widely used for follow up of CML patients during therapy or after bone marrow transplantation (BMT). We describe here a microtitre, colorimetric assay (DNA Enzyme Immunoassay, DEIA) for analysis of t(9;22) translocation after enzymatical amplification of RNA from CML patients. This assay is based on the use of a monoclonal antibody specifically reacting with double stranded DNA, i.e. with hybridized DNA. The assay represents a nonisotopic alternative to other current hybridization assays and requires no modifications of primers, probe or target DNA.

References

Dec 1, 1991·British Journal of Haematology·S Izraeli, T Lion
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·F F Chehab, Y W Kan
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G FranchiniR C Gallo
Feb 15, 1989·Analytical Biochemistry·G H KellerM M Manak
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R K SaikiH A Erlich
Dec 9, 1988·Nucleic Acids Research·A C SyvänenH Söderlund
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E S KawasakiF P McCormick
Jan 1, 1987·Methods in Enzymology·K B Mullis, F A Faloona

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Citations

Mar 1, 1995·Histopathology·L X PanP G Isaacson
Aug 20, 2010·Cellular Immunology·Saad ZakyMahmoud Rezk Abdelwahed Hussein
Jul 12, 2014·Expert Review of Molecular Diagnostics·Zonglin HuChristopher A Elkins
Mar 11, 2004·Journal of Endocrinological Investigation·N Fortunati, R Frairia

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