PMID: 2502527Apr 1, 1989Paper

Detection of beta-galactofuranosidase production by Penicillium and Aspergillus species using 4-nitrophenyl beta-D-galactofuranoside

The Journal of Applied Bacteriology
M A CousinJ H Van Boom

Abstract

An assay was developed for detecting beta-galactofuranosidase produced by Penicillium and Aspergillus spp. The substrate for the assay, 4-nitrophenyl beta-D-galactofuranoside, was synthesized from penta-O-acetyl-beta-D-galactofuranose and 4-nitrophenol by a tin chloride catalyzed reaction followed by O-deacetylation. Aspergillus spp. produced only small quantities of beta-galactofuranosidase during 30 d at 25 degrees C. Only the biverticillate Penicillium spp. (P. funiculosum, P. islandicum, P. rubrum and P. tardum) produced substantial beta-galactofuranosidase after 1-4 weeks at 25 degrees C. No extracellular antigens of these four Penicillium spp. could be detected in culture filtrates by the sandwich ELISA technique when antibodies to the extracellular beta-galactofuranoside-containing polysaccharide antigen of P. digitatum was used. Antigens to all other Penicillium and Aspergillus spp. were easily detected in their culture filtrates.

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Citations

Mar 5, 2003·Molecular and Biochemical Parasitology·Luiz Claudio MilettiRosa M de Lederkremer
Sep 24, 2011·Glycobiology·Boris TefsenFrançoise H Routier
Jan 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Erfei SongGary Sweeney
May 20, 2020·International Journal of Molecular Sciences·Mateja SeničarRichard Daniellou
Aug 1, 1993·Applied and Environmental Microbiology·G J Tsai, M A Cousin
Jun 15, 2010·Bioorganic & Medicinal Chemistry·Andrea BordoniCarla Marino

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