PMID: 2544510Jul 1, 1989

Detection of beta-thalassemia and hemoglobin E genes in Thai by a DNA amplification technique

Human Genetics
P WinichagoonS Fucharoen

Abstract

Enzymatic DNA amplification and polyacrylamide gel electrophoresis, which demonstrate different sizes of DNA fragments, were used to detect the common mutations causing beta-thalassemia and hemoglobin (Hb) E in Thai people. The 4-bp deletion at codons 41 and 42 can be detected directly by polyacrylamide gel electrophoresis and ethidium bromide staining. Whereas the nonsense mutations at codon 17 (AAG----TAG) and Hb E (GAG----AAG at codon 26) were detected after digestion of the amplified DNA with the enzymes MaeI and MnlI, respectively.

Citations

Apr 17, 1998·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·C WanapirakA Tuggapichitti
May 30, 2017·Asian Pacific Journal of Tropical Medicine·Praphat RuengthanooJatupol Kampuansai

Related Concepts

Sense Codon
DNA, Double-Stranded
DNA Restriction Enzymes
SDS-PAGE
Gene Amplification
Globin
Hemoglobin E
Abnormal Hemoglobins
Thalassemia
Oligoribonucleotide Probes

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