Detection of Biomolecular Binding Through Enhancement of Localized Surface Plasmon Resonance (LSPR) by Gold Nanoparticles.

Sensors
Hyung Min KimYong-Beom Shin

Abstract

To amplify the difference in localized surface plasmon resonance (LSPR) spectra of gold nano-islands due to intermolecular binding events, gold nanoparticles were used. LSPR-based optical biosensors consisting of gold nano-islands were readily made on glass substrates using evaporation and heat treatment. Streptavidin (STA) and biotinylated bovine serum albumin (Bio-BSA) were chosen as the model receptor and the model analyte, respectively, to demonstrate the effectiveness of this detection method. Using this model system, we were able to enhance the sensitivity in monitoring the binding of Bio-BSA to gold nano-island surfaces functionalized with STA through the addition of gold nanoparticle-STA conjugates. In addition, SU-8 well chips with gold nano-island surfaces were fabricated through a conventional UV patterning method and were then utilized for image detection using the attenuated total reflection mode. These results suggest that the gold nano-island well chip may have the potential to be used for multiple and simultaneous detection of various bio-substances.

References

Aug 31, 2002·Chemistry : a European Journal·Gregory KalyuzhnyIsrael Rubinstein
Dec 13, 2003·Analytical Chemistry·Filip FrederixGustaaf Borghs
Aug 25, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Michal LahavIsrael Rubinstein
Jan 31, 2007·Biosensors & Bioelectronics·Yong-Beom ShinSunglyul Maeng
Oct 31, 2008·Talanta·Chanda Ranjit YonzonRichard P Van Duyne

❮ Previous
Next ❯

Citations

Sep 4, 2013·Journal of Colloid and Interface Science·Jie YuanShan Zou
Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Dec 21, 2010·Journal of Colloid and Interface Science·Dongxiang LiDong Ha Kim
Mar 18, 2016·Biosensors & Bioelectronics·Na Rae JoYong-Beom Shin
Sep 9, 2015·Analytical and Bioanalytical Chemistry·Andreas HorrerMonika Fleischer
Jun 9, 2011·Chemical Reviews·Kathryn M Mayer, Jason H Hafner
Feb 22, 2012·Sensors·Taerin ChungByoungho Lee
Apr 30, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Chong LiYibing Zhao
Jun 11, 2011·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·W Paige HallRichard P Van Duyne

❮ Previous
Next ❯

Methods Mentioned

BETA
surface plasmon resonance
biosensing
chips
chip
AFM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.