Detection of biomolecular interaction between biotin and streptavidin on a self-assembled monolayer using magnetic nanoparticles

Biotechnology and Bioengineering
Atsushi ArakakiTetsuya Osaka

Abstract

For developing a magnetic bioassay system, an investigation to determine the presence of a specific biomolecular interaction between biotin and streptavidin was done using magnetic nanoparticles and a silicon substrate with a self-assembled monolayer. Streptavidin was immobilized on the magnetic particles, and biotin was attached to the monolayer-modified substrate. The reaction of streptavidin-modified magnetic particles on the biotin-modified substrate was clearly observed under an optical microscope. The magnetic signals from the particles were detected using a magnetic force microscope. The results of this study demonstrate that the combination of a monolayer-modified substrate with biomolecule-modified magnetic particles is useful for detecting biomolecular interactions in medical and diagnostic analyses.

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Citations

Jan 13, 2006·Analytical and Bioanalytical Chemistry·Tetsuya OsakaHironori Iida
Jun 19, 2008·Journal of the Royal Society, Interface·Atsushi ArakakiTadashi Matsunaga
Feb 10, 2010·Biophysical Journal·Andriy V MoskalenkoSergey V Smirnov
Oct 3, 2006·Journal of Microbiological Methods·Martin H F MeyerMichael Keusgen
Apr 3, 2016·Analytical and Bioanalytical Chemistry·Pablo Darío QuevedoUte Resch-Genger
Mar 4, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Dambarudhar MohantaRobert H Blick
Aug 20, 2015·Critical Reviews in Biotechnology·Abhilasha Singh Mathuriya
Jul 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Marina Martínez-CarmonaMaria Vallet-Regí
Aug 14, 2015·Critical Reviews in Biotechnology·Abhilasha Singh Mathuriya
Apr 29, 2010·ACS Applied Materials & Interfaces·Benjamin S FlavelAlison J Downard
Aug 26, 2010·ACS Applied Materials & Interfaces·Hidenori Mizuno, Jillian M Buriak

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