Detection of biomolecules in electrophoresis gels with salts of imidazole and zinc II: a decade of research

Electrophoresis
Lila R Castellanos-Serra, Eugenio Hardy

Abstract

The proven ability of gel electrophoresis to simultaneously resolve, in a single experiment, many components from complex biological samples, has determined its preference over a variety of well-established chromatographic methods. Therefore, procedures placed at the interface between gel separation and microanalysis have earned increasing significance with respect to the overall success of the microanalytical strategy. The first of these procedures is the detection technique. The most important requirement for compatibility with further analysis or bioapplications is that the staining method does not compromise the chemical integrity and the biological properties of micropurified biomolecules. Procedures for negative detection of proteins with metal salts that have been proven to comply with this condition have been known for about 15 years. Only recently have these procedures been extended to the field of nucleic acids and lipopolysaccharides. The focus of this review is to chronicle the development and current status of the negative or reverse stain procedure based on the in-gel reaction of imidazole with zinc salts and its applications forthe micropurification and analysis of unmodified proteins, nucleic acids and bacterial...Continue Reading

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Citations

Jun 21, 2011·Journal of Chemical Biology·Victoria J GauciJens R Coorssen
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wayne F Patton
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yasuhiro Kashino
Apr 5, 2007·Nature Protocols·Lila Castellanos-Serra, Eugenio Hardy
Jul 21, 2009·Journal of Bacteriology·Alina FaleroJavier Campos
May 24, 2006·BMC Biotechnology·Iraldo Bello-RiveroPedro López-Saura
Apr 15, 2014·Journal of Proteomics·Gabriel Padrón, Gilberto B Domont
Sep 13, 2007·Proteomics·Sylvie LucheThierry Rabilloud
Jan 30, 2008·Current Protocols in Cell Biology·Adam BrymoraPhillip J Robinson
Aug 12, 2017·The Journal of Chemical Physics·Michael KuronJoost de Graaf
Jun 5, 2010·Journal of Proteome Research·Jian-Yi PanXuan-Xian Peng

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