Detection of Bordetella avium by TaqMan real-time PCR in tracheal swabs from wildlife birds

Polish Journal of Veterinary Sciences
T StenzelA Bancerz-Kisiel

Abstract

Bordetella avium, the causing agent of bordetellosis, a highly contagious infection of the respiratory tract in young poultry, causes significant losses in poultry farming throughout the world. Wildlife birds can be a reservoir of various pathogens that infect farm animals. For this reason the studies were conducted to estimate the prevalence of Bordetella avium in wildlife birds in Poland. Tracheal swab samples were collected from 650 birds representing 27 species. The bacterial DNA was isolated directly from the swabs and screened for Bordetella avium by TaqMan real-time PCR. The assay specificity was evaluated by testing DNA isolated from 8 other bacteria that can be present in avian respiratory tract, and there was no amplification from non-Bordetella avium agents. Test sensitivity was determined by preparing standard tenfold serial dilutions of DNA isolated from positive control. The assay revealed to be sensitive, with detection limit of approximately 4.07x10^2 copies of Bordetella avium DNA. The genetic material of Bordetella avium was found in 54.54% of common pheasants, in 9.09% of Eurasian coots, in 3.22% of black-headed gulls and in 2.77% of mallard ducks. The results of this study point to low prevalence of Bordetel...Continue Reading

References

Nov 8, 2005·Journal of Clinical Microbiology·Karen B Register, Andrew G Yersin
Dec 8, 2006·Journal of Clinical Microbiology·Christoph KoidlHarald H Kessler
May 15, 2009·Biological Reviews of the Cambridge Philosophical Society·Clare McW H BenskinIan R Hartley

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Citations

Aug 29, 2019·Avian Pathology : Journal of the W.V.P.A·Rebecca KnabArne Jung
Aug 4, 2019·Tropical Animal Health and Production·Walaa Fathy Saad EldinYaser Hosny A Elewa
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Susanna GorrasiMassimiliano Fenice
Aug 21, 2021·BMC Infectious Diseases·Rui ZhangChao Feng

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