Detection of Borrelia burgdorferi DNA by polymerase chain reaction in urine specimens of patients with erythema migrans lesions

Molecular and Cellular Probes
G MercierG Lucotte

Abstract

A polymerase chain reaction (PCR) assay was developed for the detection of Borrelia burgdorferi-specific DNA in the urine of patients with erythema migrans (EM). The target for the PCR was a specific region of the flagellin gene, and DNA was extracted from urine by Chelex resin. The detection limit was 1-10 genomes of B. burgdorferi, B. garinii or B. afzelii. A prospective study was performed with 12 consecutively diagnosed patients with EM, to evaluate the PCR assay on clinical samples. Borrelia burgdorferi-specific DNA could be detected in urine specimens from the 12 patients with EM before antibiotic therapy. Five weeks after therapy all the patients were negative by PCR of urine. Results of the present study confirm that the described PCR assay is sensitive and that this sort of test allows monitoring of the efficacy of therapy in patients with early Lyme borreliosis.

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Citations

Oct 13, 2005·Acta Neurologica Scandinavica·D PíchaV Hulínský
Jan 9, 2004·Clinics in Laboratory Medicine·J Stephen Dumler
Aug 9, 2005·Clinical and Diagnostic Laboratory Immunology·Carolin RauterThomas Hartung

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