Detection of bovine herpesvirus 1 in cumulus-oocyte complexes of cows

Research in Veterinary Science
Vanessa L D Queiroz-CastroJosé D Guimarães

Abstract

Bovine herpesvirus 1 (BoHV-1) is the causative agent of infectious bovine rhinotracheitis (IBR) and is also associated with reproductive failure. This study investigated the presence of BoHV-1 in cumulus-oocyte complexes (COCs) of naturally-infected cows without clinical signs of IBR. The presence of BoHV-1 in COCs was evaluated by immunofluorescence using confocal laser scanning microscopy. Blood samples and ovaries from 82 cows that had not been vaccinated against BoHV-1 were collected for serological analysis. COCs were divided into two pools: COCs derivate from seropositive cows and from seronegative cows. Then, the samples were processed for confocal microscopy analysis. The results indicated that 61% (50/82) of cows were seropositive for BoHV-1. A total of 719 COCs were obtained from the cows and processed. None of 276 COCs from the 32 seronegative cows presented BoHV-1. However, BoHV-1 was present in the cytoplasm of cumulus cells from 158 out of 443 COCs aspirated from the seropositive cows. The detection of BoHV-1 in the COCs of seropositive cows suggests that the COCs of naturally-infected, asymptomatic cows may be infected with BoHV-1.

Citations

Jul 19, 2019·PloS One·Vanessa Lopes Dias Queiroz-CastroAbelardo Silva-Júnior
Mar 19, 2019·Theriogenology·Vanessa Lopes Dias Queiroz-CastroAbelardo Silva-Júnior
Oct 29, 2020·Theriogenology·Vanessa Lopes Dias Queiroz-CastroAbelardo Silva-Júnior

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