Detection of BRAF mutations on direct smears of thyroid fine-needle aspirates through cell transfer technique

American Journal of Clinical Pathology
Qiuying ShiHoward H Wu

Abstract

To determine the utility of the cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens. Polymerase chain reaction (PCR)-based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol-fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidectomy formalin-fixed, paraffin-embedded (FFPE) tissues on 30 cases. BRAF testing was successfully performed on 29 of 30 air-dried CTT, 27 of 30 ethanol-fixed CTT, and 27 of 30 FFPE tissues. The results exhibited 11, 13, and 13 BRAF mutations and 18, 14, and 14 wild types for the air-dried CTT, the ethanol-fixed CTT, and the FFPE tissues, respectively. The concordance rate was 96% between air-dried and ethanol-fixed CTT tissues, 88% between air-dried CTT and FFPE tissues, and 92% between ethanol-fixed CTT and FFPE tissues. PCR-based BRAF mutational testing can be reliably performed on the direct smears of the thyroid FNA specimens through the application of CTT.

References

Apr 23, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yoram CohenWilliam H Westra
Jul 27, 2004·Clinical Endocrinology·Laura FugazzolaPaolo Beck-Peccoz
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Citations

Mar 27, 2018·Cytopathology : Official Journal of the British Society for Clinical Cytology·G da Cunha SantosP Zeppa
Jul 26, 2017·Journal of Clinical Pathology·Claudio BellevicineGiancarlo Troncone
Aug 31, 2019·Thyroid : Official Journal of the American Thyroid Association·Whitney S GoldnerRichard T Kloos
Dec 20, 2019·Journal of Endocrinological Investigation·M MuzzaL Fugazzola

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