Detection of cadmium by a fiber-optic biosensor based on localized surface plasmon resonance

Biosensors & Bioelectronics
Tsao-Jen Lin, Mon-Fu Chung

Abstract

A novel transmission-based localized surface plasmon resonance (LSPR) fiber-optic probe has been developed to determine the heavy metal cadmium ion (Cd(II)) concentration. The LSPR sensor was constructed by immobilizing phytochelatins (PCs), (gammaGlc-Cys)(8)-Gly, onto gold nanoparticle-modified optical fiber (NM(Au)OF). The optimal immobilizing conditions of PCs on to the NM(Au)OF are 71.6mug/ml PCs in pH 7.4 PBS for 2h. The absorbability (change of light absorption) of the PC-functionalized NM(Au)OF sensor increases to 9% upon changing the Cd(II) level from 1 to 8ppb with a sensitivity of 1.24ppb(-1) and a detection limit of 0.16ppb. The sensor retained 85% of its original activity after nine cycles of deactivation and reactivations. In addition, the sensor retains its activity and gives reproducible results after storage in 5% d-(+)-trehalose dehydrate solution at 4 degrees C for 35 days. The dissociation constant (K(d)) of the immobilized PCs with Cd(II) was about 6.77x10(-8)M. In conclusion, the PCs-functionalized NM(Au)OF sensor can be used to determine the concentration of Cd(II) with high sensitivity.

References


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Citations

Sep 11, 2016·Chemico-biological Interactions·Aurélie JacquartNguyet-Thanh Ha-Duong
Jan 4, 2017·Sensors·Yung-Shin SunJin-Cherng Hsu
Jul 3, 2010·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Sachin K Srivastava, Banshi D Gupta
Sep 26, 2015·Applied Optics·Bárbara Rutyna HeidemannJosé Luís Fabris
Dec 30, 2014·ACS Applied Materials & Interfaces·Yanling HuLianhui Wang
Nov 13, 2012·Analytical Chemistry·Xu-Dong Wang, Otto S Wolfbeis

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