Detection of Campylobacter jejuni in food and poultry viscera using immunomagnetic separation and microtitre hybridization

Journal of Applied Microbiology
M LamoureuxR E Simard

Abstract

Thermophillic Campylobacter and Camp. jejuni were detected from samples of chicken liver, gall bladder, muscle and contaminated milk and chicken meat after an enrichment step by using immunomagnetic capture of cells with monoclonal antibody against a specific outer membrane protein of thermophilic Campylobacter. The detection of captured cells was achieved using two different hybridization methods. In one of the methods, the captured cells were lysed by guanidine isothiocyanate and the 23S rRNA was reacted with a microtitre plate-immobilized rDNA probe specific for thermophilic Campylobacter. In the other method, the captured cells were subjected to lysis by ultrasonication and the genomic DNA reacted with a microtitre plate-immobilized RNA probe specific for Camp.jejuni. Detection of the RNA-DNA hybrids formed in the wells was carried out using a monoclonal anti-RNA-DNA hybrid antibody.

Citations

Jun 29, 2010·Applied Microbiology and Biotechnology·Hari P DwivediLee-Ann Jaykus
Aug 3, 2001·International Journal of Food Microbiology·A KumarV N Bachhil
Jun 11, 1999·Current Opinion in Biotechnology·R E Mandrell, M R Wachtel
Jul 6, 2013·Canadian Journal of Microbiology·Hongsheng Huang, Beverley Phipps-Todd
Mar 17, 2015·Journal of Microbiological Methods·Mathilde H JosefsenJeffrey Hoorfar
Feb 25, 2006·Journal of Food Protection·M L HutchisonM Howell
Apr 9, 2018·Applied Microbiology and Biotechnology·Ruramayi M NzumaIrene R Grant
May 14, 2009·Journal of Food Protection·S A HeoK H Seo
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Vincenzina Fusco, Grazia Marina Quero

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.