PMID: 11337181May 5, 2001Paper

Detection of Candida spp. in blood cultures using nucleic acid sequence-based amplification (NASBA)

Diagnostic Microbiology and Infectious Disease
A BorstA C Fluit

Abstract

Candida spp. are the main causes of fungal infections in immunocompromised patients. It is known, that the routinely used automated blood culture systems may fail to detect yeasts. We therefore investigated, whether Nucleic Acid Sequence-Based Amplification (NASBA) can be used to improve the detection rate of Candida spp. in blood cultures. Culture-positive as well as negative blood cultures from patients with a proven candidaemia were analyzed, and the results of BacT/Alert monitoring were compared with the results of NASBA-based detection of yeast RNA. With the NASBA-assay, the number of positive blood cultures increased from 21% to 34%. The NASBA-assay may confirm the diagnosis and demonstrate the need for prolonged treatment. In addition it may shorten the time to detection. In summary, using NASBA for the detection of yeast RNA in blood cultures, we have shown for the first time that it is possible to improve the detection rate of yeasts in blood cultures by using amplification technology.

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Citations

Dec 6, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M S Moreira-OliveiraM L Moretti
May 15, 2002·Journal of Virological Methods·Richard A CollinsAlbert Cheung Hoi Yu
Mar 17, 2007·Medical Mycology·D H PincusS Chatellier
Feb 26, 2004·Antimicrobial Agents and Chemotherapy·Melissa D JohnsonJohn H Rex
Dec 10, 2003·Journal of Clinical Microbiology·Rangaraj SelvaranganBrad T Cookson
Jul 5, 2003·Journal of Clinical Microbiology·Younes MaaroufiFrançoise Crokaert
May 11, 2010·Current Fungal Infection Reports·L Corbin DowneyMichael Cohen-Wolkowiez
Dec 7, 2010·International Journal of Antimicrobial Agents·Michela PaolucciVittorio Sambri
Nov 30, 2004·The Lancet Infectious Diseases·Remco P H PetersChristina M J E Vandenbroucke-Grauls
Dec 31, 2002·Biochemical and Biophysical Research Communications·Richard A CollinsAlbert Cheung Hoi Yu
Jan 24, 2006·Diagnostic Microbiology and Infectious Disease·Ozay Arikan Akan, Eda Yildiz
May 20, 2003·Infectious Disease Clinics of North America·Elizabeth M O'ShaughnessyFrank G Witebsky
Nov 6, 2007·British Journal of Haematology·Penelope D Barnes, Kieren A Marr
May 3, 2013·Current Medical Research and Opinion·John R Perfect
Sep 19, 2001·Yeast
Nov 21, 2007·Journal of Clinical Laboratory Analysis·Biljana DojnovZoran Vujcić
Feb 13, 2020·Revista Do Instituto De Medicina Tropical De São Paulo·Felipe Delatorre BusserVera Lucia Teixeira de Freitas
May 14, 2019·ACS Omega·Waseem AsgharUtkan Demirci

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