Abstract
Rapid detection of candidaemia is crucial for timely antifungal chemotherapy. However, the sensitivity of automated blood culture (BC) systems has been questioned. Blind subculture might increase detection rate and possibly also reduce time to detection of candidaemia. This retrospective study aimed to evaluate the efficacy of blind subcultures in patients deemed at high risk of candidaemia. BCs were processed by the BacT/Alert BC system, and during a 5-y period (1998-2003) subculture on the third d of incubation was performed for patients selected by clinical and microbiological assessment. A total of 79,165 BCs were drawn during the study period. 2154 BCs from 285 patients were selected for subculture. 103 (4.8%) BCs from 52 patients were yeast positive; 71 were detected positive prior to the planned subculture, 25 were positive on subculture, and 7 were negative on subculture, but became positive during further incubation. The 25 BCs positive on subculture originated from 14 patients, 11 of whom had already been diagnosed with candidaemia during the previous 14 d. Thus, a primary diagnosis of candidaemia was obtained by subculture in only 3 (1.1%) of the 285 patients selected. In conclusion, in our clinical setting blind sub...Continue Reading
References
Dec 1, 1990·Journal of Clinical Microbiology·D R ReaganR P Wenzel
Sep 1, 1995·Journal of Clinical Microbiology·M L WilsonL B Reller
May 1, 1995·Journal of Clinical Microbiology·J T ShigeiE M Peterson
Apr 1, 1995·Journal of Clinical Microbiology·M P WeinsteinL B Reller
Dec 1, 1994·Annals of Surgery·D PittetR Auckenthaler
Jun 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D Abi-SaidS Vartivarian
Jun 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D W DenningR E Warren
Oct 3, 2000·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R LuzzatiE Concia
Dec 16, 2000·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A BorstA Fluit
Jun 22, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·H M BlumbergUNKNOWN National Epidemiology of Mycoses Survey(NEMIS) Study Group
Nov 13, 2002·The American Journal of Medicine·Stijn I BlotFrancis A Colardyn
Nov 26, 2002·Intensive Care Medicine·Jorge GarbinoDidier Pittet
Jun 7, 2003·Journal of Clinical Microbiology·Stanley MirrettMelvin P Weinstein
Sep 19, 2003·Intensive Care Medicine·Pierre Emmanuel CharlesBernard Blettery
Oct 9, 2003·Journal of Clinical Microbiology·Lynn L HorvathDavid P Dooley
Jan 13, 2004·Journal of Clinical Microbiology·Lynn L HorvathDuane R Hospenthal
Jan 7, 2005·Journal of Clinical Microbiology·Benjamin J GeorgeDuane R Hospenthal
Citations
Jan 29, 2011·Journal of Clinical Microbiology·Stefan RiedelKaren C Carroll
Dec 15, 2010·Critical Care Clinics·Alexander Lepak, David Andes
May 19, 2009·Diagnostic Microbiology and Infectious Disease·Julianna FernandezDavid E Nix
Aug 29, 2019·Frontiers in Cellular and Infection Microbiology·Guanlin LiLiang Ming