Detection of canine pemphigus foliaceus autoantigen by immunoblotting

Veterinary Immunology and Immunopathology
Toshiroh IwasakiY Sasaki

Abstract

The antigens targeted by autoantibodies in sera from canine patients with pemphigus foliaceus (PF) were detected by indirect immunofluorescence and Western immunoblotting. The extracted proteins from canine keratinocytes cultured in high-calcium condition for 48 h after confluency and from bovine nose epidermis were used as antigens in Western blotting. Canine keratinocytes cultured in high-calcium condition showed fluorescent deposits in intercellular spaces by incubation with sera from both canine and human pemphigus patients. By Western blotting, eight out of 16 canine PF sera recognised 160 kDa protein. 85 kDa and 120 kDa proteins were also recognised by four to five canine PF sera, respectively. The 160 kDa band, recognised by eight canine PF sera, had an identical mobility to the protein identified by a human PF serum. These results suggested that the autoantibodies in sera from canine PF recognised the 160 kDa desmosomal proteins, which may correspond to the desmoglein 1.

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Citations

Sep 10, 2003·Veterinary Immunology and Immunopathology·Koji NishifujiToshiroh Iwasaki
Oct 3, 1999·Veterinary Immunology and Immunopathology·N C Pedersen
Nov 14, 2001·Clinics in Dermatology·T Olivry, L S Chan
Aug 14, 2002·Veterinary Dermatology·Stephen D WhiteMark G Papich
Jun 15, 2010·The Journal of Veterinary Medical Science·Kaori IdeKoji Nishifuji
Nov 4, 2008·Veterinary Immunology and Immunopathology·Atsushi YabuzoeToshiroh Iwasaki
Aug 13, 2004·Veterinary Dermatology·Thierry OlivryBarbara A Atlee
Apr 19, 2003·Experimental Dermatology·Thierry OlivryReza F Ghohestani
Jan 20, 2006·Reproduction in Domestic Animals = Zuchthygiene·S Schäfer-SomiS Aslan
Nov 19, 2005·Veterinary Immunology and Immunopathology·Thierry OlivryGregg A Dean
Apr 12, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Elizabeth B SteevesDeborah M Haines
Sep 6, 2008·The Journal of Veterinary Medical Science·Atsushi YabuzoeToshiroh Iwasaki
Sep 22, 2006·Equine Veterinary Journal·V MiragliottaF Abramo

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