Sep 19, 2019

Detection of carcinoembryonic antigen using a magnetoelastic nano-biosensor amplified with DNA-templated silver nanoclusters

Jingzhe WangShengbo Sang


Here we develop a magnetoelastic (ME) nano-biosensor based on the competitive strategy for the detection of carcinoembryonic antigen (CEA). Specifically, the gold-coated ME material provided a platform and the thiolated single-stranded DNA (HS-DNA) containing half-complementary sequence towards the CEA aptamer was modified on the surface via Au-S bonding. DNA-templated silver nanoclusters (DNA-AgNCs) containing another half-complementary sequence towards the aptamer was used to amplify the signals by about 2.1 times compared to those obtained using just the aptamer. CEA aptamers as bio-recognition element was employed to link HS-DNA and DNA-AgNCs through the DNA hybridization. The CEA aptamer preferentially combined with CEA rather than hybridized with DNA. Due to the magnetostrictive nature of the ME materials, the resonant frequency of the nano-biosensor would increase along with the release of DNA-AgNCs and CEA aptamers. The modification process was demonstrated by UV-Vis spectra, X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, transmission electron microscopy (TEM) and atomic force microscope (AFM). The nano-biosensor has a linear response to the logarithmic CEA concentrations ranging from 2 pg/mL to 6.25 ng/mL,...Continue Reading

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Mentioned in this Paper

X-Ray Photoelectron Spectroscopy
Carcinoembryonic Antigen
Microscopy, Atomic Force
Scanning Probe Microscopes (Device)
Recognition (Psychology)

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