PMID: 11924702Apr 2, 2002Paper

Detection of cardiomyocyte apoptosis in forensic autopsy cases

International Journal of Legal Medicine
Masato NakatomeH Inoue

Abstract

The purpose of the present study was to determine reliable parameters for the detection of apoptotic cells for use as a diagnostic marker during the early stage of acute myocardial infarction (AMI) in forensic autopsy cases. Myocardial tissues taken from forensic autopsy cases were examined by immunohistochemical and molecular-biological methods using the terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labelling (TUNEL) and the DNA laddering methods. In cases of AMI with a time period between 2 h from onset to death and 20 h post-mortem time, the nuclei of cardiomyocytes were stained positive with the TUNEL method and DNA fragmentation of myocardial cells was detected by agarose gel electrophoresis. Similar findings were obtained in cases of carbon monoxide (CO) intoxication. However, no apoptotic cells were found in other cases such as methamphetamine (MAP) intoxication, tetrodotoxin intoxication, alcohol intoxication, asphyxia, head injury, heart injury or myocarditis. These findings suggested that it would be possible to apply TUNEL-positive cells as a diagnostic marker during the early stages of AMI.

Citations

May 12, 2007·Addiction·William Haning, Deborah Goebert
Sep 18, 2004·The American Journal of Cardiology·Ville KytöLiisa-Maria Voipio-Pulkki
Oct 13, 2012·International Journal of Cardiology·Ryohei KurodaKen-Ichi Yoshida
Jul 10, 2016·International Journal of Legal Medicine·Sara SabatassoValentin Djonov
Aug 21, 2013·The American Journal of Forensic Medicine and Pathology·Yu KakimotoKeiji Tamaki
May 13, 2017·International Journal of Legal Medicine·Sara SabatassoTony Fracasso
Mar 27, 2021·Analytica Chimica Acta·Yibo YanChuixiu Huang

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis