Detection of caveolin-3/caveolin-1/P2X7R complexes in mice atrial cardiomyocytes in vivo and in vitro.

Histochemistry and Cell Biology
C PflegerK Barth

Abstract

Caveolae and caveolins, structural components of caveolae, are associated with specific ion channels in cardiac myocytes. We have previously shown that P2X purinoceptor 7 (P2X7R), a ligand-gated ion channel, is increased in atrial cardiomyocytes of caveolin-1 knockout mice; however, the specific biochemical relationship of P2X7R with caveolins in the heart is not clear. The aim of this work was to study the presence of the P2X7R in atrial cardiomyocytes and its biochemical relationship to caveolin-1 and caveolin-3. Caveolin isoforms and P2X7R were predominantly localized in buoyant membrane fractions (lipid rafts/caveolae) prepared from hearts using detergent-free sucrose gradient centrifugation. Caveolin-1 knockout mice showed normal distribution of caveolin-3 and P2X7R to buoyant membranes indicating the importance of caveolin-3 to formation of caveolae. Using clear native-PAGE, we showed that caveolin-1, -3 and P2X7R contribute to the same protein complex in the membranes of murine cardiomyocytes and in the immortal cardiomyocyte cell line HL-1. Western blot analysis revealed increased caveolin-1 and -3 proteins in tissue homogenates of P2X7R knockout mice. Finally, tissue homogenates of atrial tissues from caveolin-3 knocko...Continue Reading

References

Apr 1, 1990·Pflügers Archiv : European journal of physiology·J L AlvarezG Vassort
Oct 4, 2000·The Journal of Biological Chemistry·M SolleC A Gabel
Dec 15, 2000·Human Molecular Genetics·Y HagiwaraT Kikuchi
Oct 13, 2001·Trends in Molecular Medicine·F GalbiatiM P Lisanti
Nov 8, 2001·Experimental Cell Research·B Razani, M P Lisanti
Jul 26, 2002·The Journal of Biological Chemistry·Scott E WoodmanMichael P Lisanti
Sep 12, 2002·Pharmacological Reviews·Babak RazaniMichael P Lisanti
Dec 28, 2002·Annals of the New York Academy of Sciences·Julie BossuytCalvin C Hale
May 1, 2004·Circulation Research·Karen A LapidosElizabeth M McNally
May 13, 2004·Journal of Neuroscience Research·Fabrizio VaccaCinzia Volonté
Oct 8, 2004·American Journal of Physiology. Cell Physiology·R X FariaLuiz Anastácio Alves
Nov 5, 2005·The Journal of Biological Chemistry·Jiliang LiCharles H Turner
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·J Michelle KahlenbergGeorge R Dubyak
Feb 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jian-Bing ShenBruce T Liang
May 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ravi C BalijepalliTimothy J Kamp
Oct 13, 2006·Molecular Pharmacology·Mark T YoungAnnmarie Surprenant
Oct 17, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jian-Bing ShenBruce T Liang
Oct 31, 2006·Journal of Molecular and Cellular Cardiology·Andrea BarbutiDario DiFrancesco
Feb 3, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hemal H PatelDavid M Roth
Feb 17, 2007·Circulation Research·Sabine MerkleStefan Engelhardt
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·Robert G Parton, Kai Simons
Nov 6, 2007·Journal of Cellular and Molecular Medicine·Woo Jung ChoEdwin E Daniel
Nov 6, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Daniela VolonteFerruccio Galbiati
Oct 22, 2008·Biochemical and Biophysical Research Communications·Annette Nicke
Oct 30, 2008·Biochemistry·Michaël RyckelynckMagali Frugier
Feb 24, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Hanny MusaHalina Dobrzynski
Mar 24, 2009·The Journal of Biological Chemistry·Miyyada BoumechacheRuth Murrell-Lagnado
Jun 2, 2009·Biochemical and Biophysical Research Communications·Vivek GargKeli Hu
Jan 8, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Kathrin BarthMichael Kasper
Feb 5, 2010·Canadian Journal of Physiology and Pharmacology·Woo Jung ChoEdwin E Daniel
Mar 25, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Wei Sun, Keli Hu
Apr 21, 2010·Cellular and Molecular Life Sciences : CMLS·Karina WeinholdKathrin Barth
Jun 22, 2010·Histochemistry and Cell Biology·K BarthM Kasper
Nov 13, 2010·Cellular and Molecular Life Sciences : CMLS·Franz BauernfeindVeit Hornung
Nov 19, 2010·The Journal of Biological Chemistry·Yogananda S MarkandeyaRavi C Balijepalli

❮ Previous
Next ❯

Citations

May 15, 2013·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Apr 2, 2013·Journal of Molecular and Cellular Cardiology·Jianding ChengJonathan C Makielski
Jun 29, 2016·Journal of Biomedical Science·Vladislav S KuzminDenis V Abramochkin
Nov 11, 2017·Cardiovascular Research·Jack P GreenHeather L Wilson
May 12, 2019·International Journal of Molecular Sciences·Karl-Philipp WesslauKathrin Barth
Dec 20, 2013·American Journal of Physiology. Cell Physiology·David Lu, Paul A Insel
Aug 24, 2019·Frontiers in Molecular Neuroscience·Robin KoppAnnette Nicke
Jan 2, 2021·International Journal of Molecular Sciences·Sun-Hee Woo, Tran Nguyet Trinh
Aug 27, 2021·Frontiers in Cardiovascular Medicine·Daniel TurnerRajan Sah

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

Atrial Filbrillation

Atrial fibrillation refers to the abnormal heart rhythm characterized by rapid and irregular beating of the atria. Here is the latest research.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.