PMID: 7539507Jul 1, 1995Paper

Detection of CD4+ T cells harboring human immunodeficiency virus type 1 DNA by flow cytometry using simultaneous immunophenotyping and PCR-driven in situ hybridization: evidence of epitope masking of the CD4 cell surface molecule in vivo

Journal of Virology
B K PattersonS M Wolinsky

Abstract

Human immunodeficiency virus type 1 (HIV-1) infection of T cells and cells of the monocyte/macrophage lineage requires a specific interaction between the CD4 antigen expressed on the cell surface and the HIV-1 external envelope glycoprotein (gp120). To study the association between HIV-1 infection and modulation of cell surface expression of the CD4 molecule in vivo, we examined the CD4+ T cells harboring proviral DNA obtained from HIV-1-infected individuals who had received no antiretroviral therapy for at least 90 days. Simultaneous immunophenotyping of CD4 cell surface expression and PCR-driven in situ hybridization for HIV-1 DNA were used to resolve the CD4+ T cells into distinct populations predicted upon the presence or absence of proviral DNA. Among the HIV-1-infected study subjects, the percentage of CD4+ T cells harboring proviral DNA ranged from 17.3 to 55.5%, with a mean of 40.5%. Cell surface fluorescent staining with anti-CD4 antibody directed against a non-gp120 binding site-related epitope (L120) or a conformation-dependent epitope of the gp120 binding site (Leu 3A) demonstrated either an equivalent or a 1.5- to 3-fold-lower cell surface staining intensity for the HIV-1 DNA-positive subpopulation relative to the ...Continue Reading

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Citations

Jun 20, 2006·Journal of Clinical Immunology·Michelle A BarronCara C Wilson
Jan 9, 2016·Current Protocols in Cytometry·Kah Teong SohPaul K Wallace
Feb 25, 2020·Current Protocols in Cytometry·Rubina PalJennifer J Stewart
Jan 12, 1999·Nature Medicine·S J BrodieS R Riddell
Jun 30, 2000·Methods : a Companion to Methods in Enzymology·J J McSharry
Nov 26, 1997·Journal of Virology·D P WooleyR C Desrosiers
Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez

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