PMID: 3230040Jan 1, 1988

Detection of cell proliferation in pig testis and intestine sections using monoclonal anti-bromodeoxyuridine antibody and immunogold silver staining

Histochemistry
D DombrowiczG Hennen

Abstract

For the first time a monoclonal antibody against 5-bromodeoxyuridine was used to detect cell proliferation in pig testis and intestine sections. The influence of several parameters such as mode of injection, addition of thymidine biosynthesis inhibitor, tissue fixation, hydrolysis and revelation was examined. The technique of choice consisted in intravenously injecting the animals with 50 mg/kg BUdR added to 10 mg/kg FUdR 2 h before tissue collection and Bouin fixation; hydrolysis of sections was performed by HC1 4N: Ethanol 70 degrees (1:1 v/v); revelation of BUdR was made by a secondary antibody linked to colloidal gold particles, followed by a silver enhancement step. The data were superior when compared to those obtained by direct immunofluorescence and by the PAP technique. The described method is convenient and sensitive, provides an intense nuclear labelling without background and allows simultaneous examination of histology. The advantages over the technique using tritiated thymidine are particularly obvious when fast screening of numerous samples is required or when new experimental protocols are developing.

References

Jan 1, 1976·Canadian Journal of Biochemistry·G JackowskiC C Liew
Dec 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger
Oct 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M De WaeleB van Camp
Jun 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G MorstynP Bhathal
Mar 1, 1986·Cell and Tissue Kinetics·D Wynford-Thomas, E D Williams
Jul 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C S HolgateC C Bird
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J Shlomai, A Kornberg

Citations

Aug 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K D Aiken, K A Roth
Sep 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K D AikenK A Roth
Dec 22, 2005·Physiological Genomics·Yuichiro NishidaJonny St-Amand

Related Concepts

Monoclonal Antibodies
NSC-38297
Cell Division Phases
Hydrolysis
Immunocytochemistry
Intestines
Testis

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