Detection of cellular micromotion by advanced signal processing.

Scientific Reports
Stephan RinnerFriedemann Reinhard

Abstract

Cellular micromotion-a tiny movement of cell membranes on the nm-µm scale-has been proposed as a pathway for inter-cellular signal transduction and as a label-free proxy signal to neural activity. Here we harness several recent approaches of signal processing to detect such micromotion in video recordings of unlabeled cells. Our survey includes spectral filtering of the video signal, matched filtering, as well as 1D and 3D convolutional neural networks acting on pixel-wise time-domain data and a whole recording respectively.

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Methods Mentioned

BETA
fluorescence imaging

Software Mentioned

Keras
Tensorflow
Inp
Adam

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