Detection of centromeres in vinblastine- and radiation-induced micronuclei of human lymphocytes using FISH with an alpha satellite pancentromeric DNA probe

Environmental and Molecular Mutagenesis
R HuberM Bauchinger

Abstract

Fluorescence in situ hybridisation (FISH) with a human alphoid satellite pancentromeric DNA probe was used to detect centromeres in micronuclei of human lymphocytes induced by gamma irradiation and by Vinblastine sulfate. In a cytokinesis-block micro-nucleus assay a dose-dependent increase of micronuclei was detected for both agents. 72-89% of vinblastine-induced micronuclei, but only 7-48% of radiation-induced micronuclei showed centromere-positive fluorescence signals. Vinblastine treatment frequencies of centromere-negative micronuclei did not increase compared to control values, nor did frequencies of centromere-positive micronuclei in irradiated lymphocytes. Since FISH with an alpha satellite DNA probe allows the direct detection of centromeric DNA sequences the spindle damaging or clastogenic effectiveness of a compound can be easily and reliably examined in a cytokinesis-block micronucleus assay in human lymphocytes.

References

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Citations

Jun 3, 2011·International Journal of Radiation Biology·Ans BaeyensAnne Vral
Jun 19, 2008·Journal of Biochemical and Molecular Toxicology·Nagarajan DevipriyaVenugopal P Menon
Jan 21, 2006·Pharmacogenetics and Genomics·Lucia MiglioreAri Hirvonen
Jan 1, 1997·International Journal of Radiation Biology·A VralL De Ridder
Apr 16, 2010·Stress : the International Journal on the Biology of Stress·Amir Mohammad MalvandiAli Moghimi
Dec 10, 1999·Micron : the International Research and Review Journal for Microscopy·Z Somosy

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