Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Scientific Reports
Chaitali SinghalJagriti Narang

Abstract

Development of platforms for a reliable, rapid, sensitive and selective detection of chikungunya virus (CHIGV) is the need of the hour in developing countries. To the best of our knowledge, there are no reports available for the electrochemical detection of CHIGVDNA. Therefore, we aim at developing a biosensor based on molybdenum disulphide nanosheets (MoS2 NSs) for the point-of-care diagnosis of CHIGV. Briefly, MoS2 NSs were synthesized by chemical route and characterized using scanning electron microscopy, transmission electron microscopy, UV-Vis spectroscopy, Raman spectroscopy and X-Ray Diffraction. MoS2 NSs were then subjected to physical adsorption onto the screen printed gold electrodes (SPGEs) and then employed for the detection of CHIGV DNA using electrochemical voltammetric techniques. Herein, the role of MoS2 NSs is to provide biocompatibility to the biological recognition element on the surface of the screen printed electrodes. The detection strategy employed herein is the ability of methylene blue to interact differentially with the guanine bases of the single and double-stranded DNA which leads to change in the magnitude of the voltammetric signal. The proposed genosensor exhibited a wide linear range of 0.1 nM to...Continue Reading

References

Dec 20, 2003·Biosensors & Bioelectronics·Fausto LucarelliMarco Mascini
Apr 16, 2010·ACS Nano·Changgu LeeSunmin Ryu
Jan 15, 2011·Physical Review Letters·Kin Fai MakTony F Heinz
Feb 22, 2012·Analytical and Bioanalytical Chemistry·Ilaria Palchetti, Marco Mascini
Sep 6, 2012·Biosensors & Bioelectronics·Manisha TyagiVinay Gupta
Apr 11, 2013·Journal of the American Chemical Society·Changfeng ZhuHua Zhang
Mar 5, 2016·Future Microbiology·Mary K McCarthy, Thomas E Morrison
Apr 3, 2016·Biosensors & Bioelectronics·Xiaorong GanXie Quan
Sep 14, 2016·ACS Nano·Alex SmolyanitskyKenneth Kroenlein
Jan 28, 2017·International Journal of Biological Macromolecules·Chaitali SinghalJagriti Narang
Apr 18, 2017·Current Opinion in Virology·Rana AbdelnabiLeen Delang
Jun 4, 2017·Biosensors & Bioelectronics·Chaitali SinghalJagriti Narang
Sep 2, 2017·Scientific Reports·Xiuxia SunJianxi Xiao

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Citations

Mar 27, 2020·Current Topics in Medicinal Chemistry·Prasad MinakshiGaya Prasad
Apr 15, 2020·Journal of the Electrochemical Society·Tugba OzerCharles S Henry
Dec 5, 2018·Biosensors & Bioelectronics·Xuanying LiZhenzhong Guo
Dec 10, 2020·Sensors·Luis Castillo-HenríquezJosé Roberto Vega-Baudrit
Jan 19, 2021·Journal of Electroanalytical Chemistry·Hüseyin Oğuzhan KayaSeda Nur Topkaya
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Katarína Nemčeková, Ján Labuda
Mar 12, 2021·Biosensors & Bioelectronics·K Yugender GoudK Vengatajalabathy Gobi
Mar 24, 2021·Scientific Reports·Jianye SuiMehdi Javanmard
Apr 30, 2021·Biosensors & Bioelectronics·Chuljin HwangJoo Hee Kim
Aug 9, 2021·Talanta·Taís Aparecida Reis CordeiroLucas Franco Ferreira
Sep 4, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Siya KamatC Jayabaskaran

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Methods Mentioned

BETA
biosensors
biosensor
X-Ray
PCR
genosensor
DropSens
scanning electron microscopy

Software Mentioned

SPGE

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