Detection of chromosomal aneuploidy by interphase fluorescence in situ hybridization in bronchoscopically gained cells from lung cancer patients

Chest
T SchenkJ Drach

Abstract

Development and progression of human malignancies involve multiple genetic changes. New techniques to distinguish neoplastic from benign diseases unequivocally with small amounts of cells as gained by bronchoscopy are needed to come closer to the goal of an early diagnosis in lung cancer. The aim of this study was to determine whether interphase fluorescence in situ hybridization (FISH) can be used to visualize chromosomal aberrations in bronchoscopically gained cells from lung cancer patients and could eventually become a complementary technique to conventional cytology. We examined 20 cancerous specimens (10 primary tumors, 10 malignant effusions) of 18 lung cancer patients by FISH with DNA probes specific for chromosomes 3, 8, 11, 12, 17, and 18. From five additional patients, endobronchial brushings and/or forceps biopsy specimens were subjected to interphase FISH analysis. In all primary tumors and malignant effusions, highly aneuploid cells were detectable by FISH. Chromosomal aberrations always consisted of gains of chromosomal signal numbers, and all chromosomes were found to be aneuploid to a similar extent. Using chromosomal aneuploidy as a marker of malignancy, material obtained by bronchoscopy was then examined for ...Continue Reading

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Citations

May 17, 2000·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·N ZojerH Huber
Nov 18, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rajbir K GillJin Jen
Mar 4, 2005·American Journal of Clinical Pathology·Lukas BubendorfMichael Tamm
Nov 17, 2012·Expert Review of Molecular Diagnostics·Jesse S VossKevin C Halling
Oct 9, 2004·International Journal of Cancer. Journal International Du Cancer·Hélène DoubreJocelyne Fleury-Feith
Aug 19, 2003·Nature Genetics·Donna G AlbertsonJoe W Gray
Jul 14, 2012·Fukushima Journal of Medical Science·Hajime ShimizuHideki Chiba
Mar 31, 2017·Oncology Letters·Madiha KanwalYi Cao
Feb 5, 2000·Seminars in Surgical Oncology·E M TolozaS G Swisher

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