DOI: 10.1101/507681Dec 31, 2018Paper

Detection of circulating extracellular mRNAs by modified small RNA-sequencing analysis

BioRxiv : the Preprint Server for Biology
Kemal Marc AkatThomas Tuschl


Extracellular mRNAs (ex-mRNAs) potentially supersede extracellular miRNAs (ex-miRNAs) and other RNA classes as biomarkers. Here, we present a comprehensive extracellular RNA (exRNA) study in human blood circulation based on conventional small RNA-sequencing (sRNA-seq) and sRNA-seq after T4 polynucleotide kinase (PNK) end-treatment of total exRNA isolated from serum and platelet-poor EDTA, ACD, and heparin plasma. Applying strict criteria for read mapping and annotation, we found that compared to conventional sRNA-seq PNK-treatment increased the detection of informative ex-mRNAs reads up to 50-fold. Based on captured ex-mRNAs from healthy individuals, we concluded that the exRNA pool is dominated by hematopoietic cells and platelets, with additional contribution from the liver. About 60% of the 15- to 42-nt long reads originated from the coding sequences, in a pattern reminiscent of ribosome-profiling studies for high abundance transcripts. Blood sample type had a considerable influence on the exRNA profile. The number of detected distinct ex-mRNA transcripts ranged from on average ~350 to 1100 in the different plasma types. In serum, additional transcripts from neutrophils and hematopoietic cells increased this number to ~2300....Continue Reading

Related Concepts

Biological Markers
Blood Circulation
Blood Platelets
Polynucleotide 5'-Hydroxyl-Kinase
RNA, Messenger

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
Y Ishizaki, N Hiraoka
European Heart Journal. Acute Cardiovascular Care
Vince C de HoogArend Mosterd
Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
Yang LiDavid T Wong
© 2021 Meta ULC. All rights reserved