PMID: 9189103Jun 1, 1997Paper

Detection of circulating tumour cells and nodal metastasis by reverse transcriptase-polymerase chain reaction technique

The British Journal of Surgery
L S WongI A Fraser

Abstract

In the search for occult metastases in lymph nodes or circulating tumour cells, a reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to detect tumour-specific splice variants of the transcript of the CD44 gene. The assay was highly sensitive and could detect ten tumour cell per 10(5) leucocytes. RNA was purified from peripheral blood (n = 24) and regional lymph nodes (n = 14) from patients with colorectal cancer. Complementary DNA was made and amplified using primers specific for the CD44 gene. Southern blotting with exon-specific probes was used to enhance the sensitivity. Tumour cells were detected in peripheral blood samples in four patients and lymph nodes in nine, in one of whom conventional histology had not detected tumour cells. This technique may be useful in the early diagnosis of primary or metastatic tumours, in assessing prognosis and in detecting residual disease after treatment.

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G R ScreatonJ I Bell
Apr 1, 1991·Cancer·A M CohenE R Sigurdson
Mar 1, 1997·The British Journal of Surgery·L S WongI A Fraser

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Citations

Dec 18, 2001·Seminars in Surgical Oncology·P Kienle, M Koch
Nov 24, 1999·Diseases of the Colon and Rectum·D García-OlmoJ Cifuentes
Jan 8, 2003·Pathology Oncology Research : POR·József TímárLászló Kopper
Apr 7, 2005·Diseases of the Colon and Rectum·A E BraatR A E M Tollenaar
May 5, 2001·Oral Oncology·T Sumida, H Hamakawa
Oct 2, 2001·The British Journal of Surgery·G TsavellasT G Allen-Mersh
Aug 22, 2000·The British Journal of Dermatology·L Brochez, J M Naeyaert
Apr 23, 2005·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Farid E Ahmed
Feb 26, 2005·International Seminars in Surgical Oncology : ISSO·Gary AtkinIan Mitchell
Jun 10, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Evi N Debruyne, Joris R Delanghe
Oct 13, 1998·Cancer Treatment Reviews·N Beechey-Newman
Apr 27, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Archangel Levi Omara-OpyeneKenneth A Iczkowski

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