PMID: 9520029Mar 31, 1998

Detection of clonal T-cell receptor gamma chain gene rearrangements by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE) in archival specimens from patients with early cutaneous T-cell lymphoma: correlation of histologic findings with PCR/DGGE

Journal of the American Academy of Dermatology
J TokA Y Matsushima

Abstract

Early stages of cutaneous T-cell lymphoma (CTCL) may be difficult to distinguish from benign inflammatory dermatoses by routine histologic examination. Our purpose was to determine whether clonal rearrangements of the T-cell receptor (TCR) gamma gene by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE) could be detected in the early stages of CTCL and to correlate these findings with conventional histopathology. A total of 39 specimens from 12 patients with CTCL were obtained. The slides were evaluated independently by three dermatopathologists, and categorized into three groups: nondiagnostic, suggestive of CTCL, and diagnostic of CTCL. Of the 39 specimens, 33 were tested by PCR/DGGE by means of GC-clamped primers for clonal rearrangement of the TCR gamma gene. The histologic evaluation of the 12 cases showed a significant variation among the three dermatopathologists. The correlation of PCR/DGGE with routine histology was as follows: Clonal TCR gamma gene rearrangements were demonstrated in 73% of the specimens nondiagnostic for CTCL, 71% of those suggestive of CTCL, and 74% of those diagnostic of CTCL. Clonal TCR gamma gene rearrangements may be detected in patients with early CTCL, even when t...Continue Reading

Citations

Feb 13, 2003·International Journal of Dermatology·Zahra Safee NaraghiYahya Dowlati
Aug 19, 2000·The American Journal of Dermatopathology·S KohlerJ L Zehnder
May 31, 2003·The American Journal of Dermatopathology·Earl Glusac
Sep 19, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Nicolas OrtonneJanine Wechsler
Feb 11, 2010·The American Journal of Dermatopathology·Gerardo FerraraCatherine M Stefanato
Jul 31, 2014·Archives of Pathology & Laboratory Medicine·Aaron C ShaverAnnette S Kim
Mar 1, 2016·American Journal of Clinical Dermatology·Sumana Devata, Ryan A Wilcox
Aug 8, 2007·The Australasian Journal of Dermatology·Christopher C I FooSuat-Hoon Tan
Nov 29, 2005·Journal of the American Academy of Dermatology·Nicola PimpinelliInternational Society for Cutaneous Lymphoma
Sep 7, 2000·Ceskoslovenská patologie·F FakanS Námĕstek
Nov 30, 2016·Experimental Dermatology·Brittany DulmageOleg E Akilov
Sep 5, 2002·Journal of Cutaneous Pathology·Niels HolmChristian A Sander
May 23, 2009·American Journal of Clinical Pathology·Ingerlisa W MattochUma Sundram
May 28, 2015·Parasitology Research·Milica ArsenovićMilan Stefanović
Sep 2, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Arturo MolinaAuayporn Nademanee
Jun 1, 2001·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·K DadejI Gòrska-Flipot
Jul 16, 2009·Journal of Cutaneous Pathology·Paul S FurmanczykZsolt B Argenyi
Sep 1, 1999·Seminars in Cutaneous Medicine and Surgery·M C Garzon
Mar 16, 2013·Pediatric Dermatology·Angel Santos-BrizMarcos González-Díaz

Related Concepts

Clone Cells
SDS-PAGE
Malignant Neoplasm of Skin
Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
Nested Polymerase Chain Reaction
T-Cell Lymphoma

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