Detection of Clostridium botulinum in liquid manure and biogas plant wastes

Folia Microbiologica
J NeuhausMonika Krüger

Abstract

Biogas plants have been considered as a source for possible amplification and distribution of pathogenic bacteria capable of causing severe infections in humans and animals. Manure and biogas wastes could be sources for spore-forming bacteria such as Clostridium botulinum. In the present study, 24 liquid manure and 84 biogas waste samples from dairies where the majority of the cows suffered from chronic botulism were investigated for the presence of botulinum neurotoxins (BoNT) and C. botulinum spores. The prevalence of BoNT/A, B, C, D, and E in biogas wastes was 16.6, 8.3, 10.7, 7.1, and 10.8 %, respectively, while in manure, the prevalence was 0.0, 0.0, 0.0, 8.3, and 4.1 %, respectively. After enrichment of samples in reinforced cultural medium, they were tested for C. botulinum BoNT/A, B, C, D, and E using ELISA (indirect C. botulinum detection). The prevalence of C. botulinum type A, B, C, D, and E samples in biogas wastes was 20.2, 15.5, 19, 10.7, and 34.8 %, respectively, while the prevalence in liquid manure was 0.0, 0.0, 0.0, 8.3, and 12.5 %, respectively. In conclusion, the occurrence of BoNT and C. botulinum spores in biogas waste of diseased animals indicates an increased and underestimated hygienic risk. Application...Continue Reading

References

Oct 1, 1994·International Journal of Systematic Bacteriology·M D CollinsJ A Farrow
Feb 6, 2003·International Journal of Food Microbiology·Maria DahlenborgPeter Rådström
Apr 21, 2006·Progress in Molecular and Subcellular Biology·Bernhard Schink
Jul 6, 2014·Folia Microbiologica·Jürgen NeuhausMonika Krüger

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Citations

Nov 25, 2016·Animal : an International Journal of Animal Bioscience·P A HarrisI Vervuert
Nov 18, 2018·Folia Microbiologica·Lisa-Maria MauerhoferSimon K-M R Rittmann

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