Detection of Clostridium botulinum toxin by local paralysis elicited with intramuscular challenge.

Applied Microbiology
H SugiyamaB R Dasgupta

Abstract

Clostridium botulinum toxin can be identified by a characteristic, acute local paralysis that follows its injection into the gastrocnemius ("calf" muscle) of mice. The local botulism can be elicited with slightly less than one-tenth the toxin amount that is needed to kill mice by the intraperitoneal (i.p.) challenge route. The practical sensitivities of the intramuscular (i.m.) versus i.p. tests are about equal, however, because maximum sample volume injectable i.m. is 0.1 ml as compared to the 0.5-ml range that can be given i.p. i.m. injection of 10 or more mouse i.p. mean lethal doses causes paralysis in about 1 h, and an i.m. injection of about 0.5 i.p. mean lethal doses causes paralysis in 3 to 4 h. Toxin neutralization by homologous type of antitoxin only can be demonstrated with an incubated mixture of toxin and antitoxin. Although not as convenient as the i.p. method for routine use to detect botulinum toxin, the i.m. method has characteristics which could make it a useful supplement to the presently accepted i.p. procedure.

References

Jun 1, 1960·The Journal of Physiology·S THESLEFF

❮ Previous
Next ❯

Citations

Dec 24, 1997·Toxicon : Official Journal of the International Society on Toxinology·L B PearceA Gupta

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Related Papers

Journal of Toxicology. Clinical Toxicology
William H RichardsonSaralyn R Williams
Archivos de la Sociedad Española de Oftalmología
F Aguirre-BalsalobreJ R Hueso-Abancens
© 2022 Meta ULC. All rights reserved