PMID: 8192150Jul 1, 1994

Detection of common deletional alpha-thalassemia-2 determinants by PCR

American Journal of Hematology
E Baysal, T H Huisman

Abstract

We have developed a rapid approach to detect the two most common alpha-thalassemia-2 (alpha-thal-2) determinants by the polymerase chain reaction (PCR) technique, which takes a few hours to complete. Specific oligonucleotides selectively amplify appropriate segments of the chromosome with the deletion and the normal chromosome under identical experimental conditions, and the products are identified by electrophoresis on 1.5% agarose. Characterization of the two most prevalent types of the -alpha 3.7 determinant [-alpha 3.7(I) and -alpha 3.7(II)] can be made by Apa I digestion of the PCR product. Two types of alpha-thal-2 determinants, -alpha 3.7 and -alpha 4.2, were tested in numerous samples from various parts of the world. This approach is believed to provide a cost-effective way to screen large numbers of blood samples in a relatively short time and can be used to identify alpha-thal-2 heterozygotes and homozygotes and compound heterozygotes (-alpha 3.7/-alpha 4.2) in populations where such alpha-gene defects are shown to exist at high frequencies.

References

Jan 1, 1979·Hemoglobin·P WasiP Winichagoon
May 1, 1992·British Journal of Haematology·D K BowdenD R Higgs
Nov 1, 1991·American Journal of Hematology·J B ZhaoT H Huisman
Jan 1, 1989·Hemoglobin·S A Liebhaber
Jan 1, 1988·Hemoglobin·R J TrentM Hertzberg
Dec 1, 1988·Journal of Medical Genetics·V J BuckleD J Weatherall
Jan 1, 1985·Annals of the New York Academy of Sciences·Y W Kan
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E A ZimmerA C Wilson
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·D J Weatherall

Citations

Dec 1, 1996·Prenatal Diagnosis·J M Old
May 22, 2001·Prenatal Diagnosis·M KleanthousM Angastiniotis
Nov 30, 2006·Journal of Applied Genetics·Vaikam H SankarSarita Agarwal
Oct 4, 2011·Indian Journal of Clinical Biochemistry : IJCB·Vijay S BhatPatnam Rajagopalan Krishnaswamy
Jan 2, 2014·Indian Journal of Clinical Biochemistry : IJCB·Sanjay PandeyR Saxena
Dec 3, 1999·British Journal of Haematology·R L NagelM E Fabry
May 13, 1999·Tropical Medicine & International Health : TM & IH·F P MockenhauptU Bienzle
Dec 29, 2000·Journal of Pediatric Hematology/oncology·P WinichagoonP Wasi
May 29, 2004·Journal of Pediatric Hematology/oncology·Renu Gupta, Adekunle D Adekile
Nov 2, 2011·Journal of Pediatric Hematology/oncology·Hernan SabioF Kutlar
Oct 1, 1994·British Journal of Haematology·T P MolchanovaT H Huisman
Mar 1, 1995·British Journal of Haematology·E BaysalT H Huisman
Aug 17, 2005·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Adekunle D AdekileF Kutlar
Aug 17, 2005·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Mohammad Haider, Adekunle D Adekile
Jun 2, 2007·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Adekunle D AdekileJalaja Sukumaran
Mar 8, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Amal LouahabiDiane Maisin
Nov 23, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Luksana MakonkawkeyoonSanit Makonkawkeyoon
Oct 24, 2009·Haematologica·Luksana MakonkawkeyoonSanit Makonkawkeyoon
Dec 17, 2009·European Journal of Applied Physiology·Imed TouhamiDaniel Le Gallais
Nov 18, 2015·Hemoglobin·Samaneh FarashiHossein Najmabadi
Dec 10, 2008·Hemoglobin·Khodamorad ZandianHossein Najmabadi
May 14, 2008·Hemoglobin·Hamida Mesbah-AmrounJacques Elion
Apr 18, 2009·Hemoglobin·Ahmad TamaddoniHossein Najmabadi
Jan 22, 2011·Hemoglobin·Saedieh EbrahimkhaniHossein Najmabadi
May 9, 2007·Hemoglobin·Aisha Al-ObaidliR Krishnamoorthy
May 9, 2007·Hemoglobin·M Akif Cürük
Aug 7, 2009·Hemoglobin·Aisha Al-ObaidliR Krishnamoorthy
Aug 7, 2009·Hemoglobin·Valeh HadaviHossein Najmabadi
Sep 4, 2015·Hemoglobin·Samaneh FarashiHossein Najmabadi
Nov 3, 2015·Expert Review of Hematology·Samaneh FarashiAzita Azarkeivan
May 1, 2010·Expert Opinion on Medical Diagnostics·John Old, Shirley Henderson
Jun 1, 2005·Hemoglobin·Pimlak CharoenkwanTorpong Sanguansermsri
Feb 9, 2002·American Journal of Hematology·Isabel GonçalvesLeonor Osório-Almeida
Apr 9, 2005·British Journal of Haematology·Anjali Angelika SarkarUma B Dasgupta
Oct 16, 2015·Blood Cells, Molecules & Diseases·Samaneh Farashi, Hossein Najmabadi
Mar 4, 2006·British Journal of Haematology·Francisco J C PereiraLuísa Romão
Aug 5, 2006·Translational Research : the Journal of Laboratory and Clinical Medicine·Jing-Zhong LiuBai Xiao
Nov 15, 2011·Hemoglobin·Erol Baysal
Jun 1, 2016·The Indian Journal of Medical Research·Somphon PharephanLuksana Makonkawkeyoon
Oct 17, 2013·PloS One·Edward KabyemelaPatrick E Duffy
Sep 12, 2012·São Paulo Medical Journal = Revista Paulista De Medicina·Sanjay PandeyRenu Saxena
Sep 6, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M R WenningM F Sonati
Sep 1, 1995·Hemoglobin·L H GuT H Huisman
May 1, 1997·Hemoglobin·S el-Kalla, A R Mathews
Jan 7, 1998·Hemoglobin·S el-Kalla, A R Mathews
Jan 1, 1997·Hemoglobin·C OnerC Altay
May 18, 2000·American Journal of Hematology·R RaghupathyAdekunle D Adekile
Feb 9, 2002·American Journal of Hematology·Adekunle D AdekileS Al-Mohannadi
Oct 1, 1996·American Journal of Hematology·A C KattamisP Fortina
Jan 7, 2017·Journal of Pediatric Hematology/oncology·Adekunle D AdekileDiana Thomas
Aug 2, 2001·Hemoglobin·Adekunle D Adekile
Jul 12, 2002·American Journal of Hematology·Adekunle D AdekileA Moosa
Jun 26, 2002·European Journal of Human Genetics : EJHG·Samia Zertal-ZidaniR Krishnamoorthy
Sep 28, 2002·American Journal of Hematology·S SvastiSuthat Fucharoen
Jun 27, 2003·American Journal of Hematology·Wafaa LemsaddekLeonor Osório-Almeida
Jul 25, 2003·American Journal of Hematology·Rajaa MaroufAdekunle D Adekile
Jan 8, 2014·Journal of Clinical Laboratory Analysis·Sanjay PandeyRenu Saxena
Nov 9, 2011·The Korean Journal of Hematology·Sanjay PandeyR Saxena
Apr 26, 2005·American Journal of Hematology·Adekunle D AdekileA D Adekile
Dec 14, 2005·The Journal of Obstetrics and Gynaecology Research·Yong-Chui WeeJin-Ai Mary Anne Tan
Sep 4, 2019·Journal of Clinical Laboratory Analysis·Seyed Mohammad Bagher Hashemi-SotehAhmad Tamadoni
Oct 24, 2002·Blood·David H K ChuiVivian Chan
Aug 6, 1998·American Journal of Hematology·V Oron-KarniD Rund
Dec 13, 2006·Clinical Chemistry·Sherry Sze Yee HoMahesh Choolani
Jan 17, 2002·Hemoglobin·S SvastiS Fucharoen
Feb 27, 2003·Hemoglobin·Maryam NeishaburyHossein Najmabadi
Sep 1, 1996·American Journal of Hematology·Adekunle D AdekileA Owunwanne
Dec 5, 1998·American Journal of Hematology·R GalanelloA Cao
Jul 31, 2010·Pediatric Hematology and Oncology·Azita AzarkeivanNajmabadi Hossein
May 31, 2019·Hemoglobin·Adekunle D AdekileDiana Thomas
Aug 25, 2020·BMC Medical Genetics·Adekunle D AdekileMohammad Haider
Jun 1, 2013·International Journal of Laboratory Hematology·C L Harteveld
Jun 6, 1998·Pediatric Hematology and Oncology·S el-Kalla, E Baysal
Apr 10, 2002·Hemoglobin·Nélida I NogueraAngela C Milani
Jul 1, 2012·Indian Journal of Clinical Biochemistry : IJCB·S PandeyR Saxena
Jul 15, 2018·International Journal of Hematology·Kesara Nittayaboon, Chamnong Nopparatana
May 21, 1999·Hemoglobin·D ItchayananS Fucharoen
Apr 3, 2021·Annals of Hematology·Rajaa MaroufOlusegun A Mojiminiyi

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DNA Sequence
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Alpha-Thalassemia
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