Detection of common flagella antigen in Bacillus cereus by monoclonal antibody

Microbiology and Immunology
T MikamiM Suzuki

Abstract

Bacillus cereus has been classified into 23 types by immunochemical analyses of flagella antigen, but a common antigenic determinant of flagella had not been determined. When the immunochemical method of classification had changed from "agglutination method" to "enzyme-linked immunosorbent assay (ELISA)," the cross-reactivity between each flagella type was increased. These results indicated that the application of ELISA method would enable detection of the common antigenic determinant of B. cereus. Therefore, we attempted to make monoclonal antibody against common flagella antigen that could be detected by ELISA. Monoclonal antibody provided was specific for B. cereus (H-1 to H-23) and did not show the cross-reactivity with Escherichia coli NIH and Bacillus subtilis.

References

Jul 1, 1977·Journal of General Microbiology·A M LawnF Orskov
Nov 1, 1975·Journal of Medical Microbiology·A J Taylor, R J Gilbert
Sep 1, 1986·Chemical & Pharmaceutical Bulletin·T MikamiM Suzuki
Jan 1, 1971·Journal of Bacteriology·M L DePamphilis, J Adler
Jul 14, 1968·Journal of Molecular Biology·S AsakuraT Iino
Jan 1, 1981·Pharmacology & Therapeutics·P C Turnbull
Feb 1, 1965·Journal of Molecular Biology·J LOWY, J HANSON

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Citations

Mar 1, 1993·FEMS Microbiology Letters·T MurakamiM Suzuki

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