Abstract
PCR typing methods were used to assess the presence of plasmids of the incompatibility (Inc) groups IncP, IncN, IncW, IncQ and rolling circle plasmids of the pMV158 type in total DNA extracts from anthropogenic soils from India and Germany. Ten different soils from two different locations in Germany, the urban park Berlin Tiergarten and the abandoned sewage field Berlin-Buch, and from four different locations in India were analysed. PCR amplification of the total DNA extracts revealed the prevalence of IncP-specific sequences in Berlin Buch and Indian soil samples. The detected IncP plasmids contained at least one transfer function, the origin of transfer, oriT. In contrast to IncP-specific sequences, IncQ, IncN, IncW and pMV158-specific sequences were never detected. The presence of ampC, tet (O), ermB, SHV-5, mecA, and vanA antibiotic resistance genes was also tested. Three Indian soil samples irrigated with wastewater contained the ampC gene, whereas the other resistance genes were not found in any of the samples. Detection of IncP trfA2 and oriT sequences by PCR amplification and hybridization is a clear indication that IncP plasmids are prevalent in these habitats. Exogenous plasmid isolation revealed conjugative plasmids ...Continue Reading
References
May 3, 1990·Nature·D M WardM M Bateson
Jan 1, 1991·Critical Reviews in Microbiology·V Braun, T Focareta
Nov 1, 1988·Applied and Environmental Microbiology·M J BaleJ C Fry
Dec 8, 1983·Nature·N Datta, V M Hughes
Nov 7, 1996·Gene·S Silver
Mar 7, 1997·Journal of Molecular Biology·L M Guzmán, M Espinosa
Jul 8, 1999·Molecular & General Genetics : MGG·E GrohmannM Espinosa
Mar 4, 2000·Inflammatory Bowel Diseases·T A Ullman
Oct 31, 2000·Applied and Environmental Microbiology·K SmallaE Tietze
Jul 12, 2001·Research in Microbiology·V C Nwosu
May 30, 2002·Analytical Chemistry·Gerd HamscherHeinz Nau
Oct 25, 2002·International Journal of Environmental Health Research·Nwadiuto EsiobuJoseph Ike
Nov 8, 2002·Bioresource Technology·Asma AleemAbdul Malik
Feb 8, 2003·Bioresource Technology·Akbar NawabAbdul Malik
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Elisabeth GrohmannManuel Espinosa
Jul 2, 2003·Mutation Research·Asma Aleem, Abdul Malik
Aug 22, 1964·Nature·J K FAULKNER, D WOODCOCK
Sep 23, 2003·Research in Microbiology·Marie-Eve GstalderMartine Couturier
Sep 23, 2003·Journal of Microbiological Methods·Uta BöckelmannElisabeth Grohmann
Dec 11, 2003·Environment International·Yvonne AgersøLars Bogø Jensen
Feb 21, 2004·Water Research·Maria AlexandrinoUlrich Szewzyk
Aug 13, 2004·Environmental Microbiology·Christian S RiesenfeldJo Handelsman
Aug 23, 2005·Folia Microbiologica·R FraniczekK Szufnarowski
Jan 21, 2006·Science·Vanessa M D'CostaGerard D Wright
Feb 1, 2006·Plasmid·Karen Mine HaradaKousaku Murata
Apr 7, 2006·Microbial Ecology·Heike SchmittWillem Seinen
Jun 1, 2006·FEMS Microbiology Letters·Luca Guardabassi, Yvonne Agersø
Sep 2, 2006·Microbiology·Anthony S HainesJohn C Fry
Sep 19, 2006·Applied and Environmental Microbiology·Kornelia SmallaChristopher M Thomas
Dec 15, 2006·Bioresource Technology·Mohd Ikram Ansari, Abdul Malik
Feb 15, 2007·Environmental Microbiology·Andrea J PattersonKaren P Scott
Feb 27, 2007·Plasmid·Anthony S HainesChristopher M Thomas
Citations
Jan 9, 2009·Applied Microbiology and Biotechnology·Xu-Xiang ZhangHerbert H P Fang
May 29, 2010·Applied Microbiology and Biotechnology·Chang Ding, Jianzhong He
Sep 21, 2010·Environmental Monitoring and Assessment·Abdul Malik, Asma Aleem
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Reshma AnjumAbdul Malik
Feb 5, 2011·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Chih-Chauan KaoZhi-Yuan Shi
Mar 27, 2010·The Journal of General and Applied Microbiology·Kazunari UshidaHiroshi Kanda
Jul 6, 2014·Journal of Hazardous Materials·Baowei ChenXiangdong Li
Jan 24, 2016·The Science of the Total Environment·Jekaterina JutkinaD G Joakim Larsson
Dec 26, 2015·FEMS Microbiology Ecology·Simone DealtryKornelia Smalla
Dec 17, 2009·Research in Microbiology·Alexandra MouraAntónio Correia
Jun 13, 2013·Environmental Microbiology Reports·Takahiro SegawaKazunari Ushida
May 9, 2012·International Journal of Evolutionary Biology·Matti Jalasvuori
Sep 28, 2013·Environmental Pollution·Feng-Hua WangYong-Guan Zhu
Oct 3, 2019·Recent Patents on Biotechnology·Manzar AlamSyed Sayeed Ahmad
Apr 12, 2016·Journal of Environmental Quality·Elizabeth LubyAmy Pruden
Apr 29, 2009·Journal of Environmental Quality·Joanne C Chee-SanfordRustam I Aminov
Feb 3, 2011·Journal of Environmental Quality·Tatsuya AkiyamaMary C Savin