Detection of conjugative plasmids and antibiotic resistance genes in anthropogenic soils from Germany and India

FEMS Microbiology Letters
A MalikElisabeth Grohmann

Abstract

PCR typing methods were used to assess the presence of plasmids of the incompatibility (Inc) groups IncP, IncN, IncW, IncQ and rolling circle plasmids of the pMV158 type in total DNA extracts from anthropogenic soils from India and Germany. Ten different soils from two different locations in Germany, the urban park Berlin Tiergarten and the abandoned sewage field Berlin-Buch, and from four different locations in India were analysed. PCR amplification of the total DNA extracts revealed the prevalence of IncP-specific sequences in Berlin Buch and Indian soil samples. The detected IncP plasmids contained at least one transfer function, the origin of transfer, oriT. In contrast to IncP-specific sequences, IncQ, IncN, IncW and pMV158-specific sequences were never detected. The presence of ampC, tet (O), ermB, SHV-5, mecA, and vanA antibiotic resistance genes was also tested. Three Indian soil samples irrigated with wastewater contained the ampC gene, whereas the other resistance genes were not found in any of the samples. Detection of IncP trfA2 and oriT sequences by PCR amplification and hybridization is a clear indication that IncP plasmids are prevalent in these habitats. Exogenous plasmid isolation revealed conjugative plasmids ...Continue Reading

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Citations

Jan 9, 2009·Applied Microbiology and Biotechnology·Xu-Xiang ZhangHerbert H P Fang
May 29, 2010·Applied Microbiology and Biotechnology·Chang Ding, Jianzhong He
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Reshma AnjumAbdul Malik
Feb 5, 2011·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Chih-Chauan KaoZhi-Yuan Shi
Mar 27, 2010·The Journal of General and Applied Microbiology·Kazunari UshidaHiroshi Kanda
Jan 24, 2016·The Science of the Total Environment·Jekaterina JutkinaD G Joakim Larsson
Jun 13, 2013·Environmental Microbiology Reports·Takahiro SegawaKazunari Ushida
Sep 28, 2013·Environmental Pollution·Feng-Hua WangYong-Guan Zhu
Apr 29, 2009·Journal of Environmental Quality·Joanne C Chee-SanfordRustam I Aminov
Feb 3, 2011·Journal of Environmental Quality·Tatsuya AkiyamaMary C Savin

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