Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers

PloS One
Asma IqbalMaxim V Berezovski

Abstract

There are currently no standard methods for the detection of Cryptosporidium spp., or other protozoan parasites, in foods, and existing methods are often inadequate, with low and variable recovery efficiencies. Food testing is difficult due to the low concentrations of parasites, the difficulty in eluting parasites from some foods, the lack of enrichment methods, and the presence of PCR inhibitors. The main objectives of the present study were to obtain DNA aptamers binding to the oocyst wall of C. parvum, and to use the aptamers to detect the presence of this parasite in foods. DNA aptamers were selected against C. parvum oocysts using SELEX (Systematic Evolution of Ligands by EXponential enrichment). Ten rounds of selection led to the discovery of 14 aptamer clones with high affinities for C. parvum oocysts. For detecting parasite-bound aptamers, a simple electrochemical sensor was employed, which used a gold nanoparticle-modified screen-printed carbon electrode. This aptasensor was fabricated by self-assembling a hybrid of a thiolated ssDNA primer and the anti- C. parvum aptamer. Square wave voltammetry was employed to quantitate C. parvum in the range of 150 to 800 oocysts, with a detection limit of approximately 100 oocyst...Continue Reading

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Dion A DanielsLarry Gold
Mar 21, 2006·Trends in Parasitology·L SavioliA Thompson
Jun 19, 2008·Journal of the American Chemical Society·Maxim V BerezovskiSergey N Krylov
Jan 2, 2009·Biosensors & Bioelectronics·Edith Torres-Chavolla, Evangelyn C Alocilja
Jun 12, 2010·Nature Protocols·Kwame SefahWeihong Tan
Jan 5, 2011·Emerging Infectious Diseases·Elaine ScallanPatricia M Griffin
Mar 15, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Tatjana CoklinBrent R Dixon
Nov 8, 2011·Journal of Food Protection·Ebo Budu-AmoakoJ T McClure
Dec 19, 2012·Annali Dell'Istituto Superiore Di Sanità·Rachel M Chalmers
Apr 10, 2013·Trends in Parasitology·Rachel M Chalmers, Frank Katzer
Aug 7, 2013·Advances in Biochemical Engineering/biotechnology·Mahmoud Labib, Maxim V Berezovski

❮ Previous
Next ❯

Citations

Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Oct 28, 2016·Biotechnology Journal·Laura SutarlieXiaodi Su
Jan 20, 2018·Parasitology Research·Shahira A Ahmed, Panagiotis Karanis
May 29, 2020·Biomédica : revista del Instituto Nacional de Salud·Juan David Ospina
Dec 4, 2018·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)Lucy Robertson
Nov 30, 2018·Genes·Juan David Ospina-VillaLaurence A Marchat
Aug 25, 2020·Journal of Water and Health·Hercules SakkasPanagiotis Karanis
Jun 27, 2020·Applied Microbiology and Biotechnology·Fernanda Raquel Wust SchmitzDachamir Hotza
May 5, 2020·Biosensors & Bioelectronics·Ellen Cesewski, Blake N Johnson
Mar 9, 2021·Frontiers in Microbiology·Mariam Saad, Sebastien P Faucher
Jun 4, 2021·Veterinary Research·Solène NiederlenderGrégory Karadjian
Jul 30, 2021·Journal of Hazardous Materials·George LukaMina Hoorfar

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
biosensor
electrophoresis
flow cytometry
enzyme-linked immunosorbent assay

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.