Detection of CSF 14-3-3 Protein in Sporadic Creutzfeldt-Jakob Disease Patients Using a New Automated Capillary Western Assay

Molecular Neurobiology
A FourierI Quadrio

Abstract

Sporadic Creutzfeldt-Jakob disease (sCJD) is a fatal rapidly progressive dementia. The detection of 14-3-3 protein in cerebrospinal fluid (CSF) is included in the WHO diagnostic criteria for the pre-mortem diagnosis of CJD. The aim of this study is to assess CSF 14-3-3 protein analytical and diagnostic performances using a new automated capillary Western technology (Simple Western technology-SW). For the validation of this assay, samples from a cohort of 268 patients suspected from sCJD were analyzed: 77 sCJD (including 40 definite sCJD) and 191 non-CJD samples were tested using both SW and the current Western Blot (WB) assays. Automated capillary Western determination provided better analytical performances than WB with a lower intra- and inter-assay variability. Analytical interferences such as hemolysis and high total protein concentration known to lead to false positive WB results were also assessed using SW assay: unfortunately, these interferences still remain confounders of CSF 14-3-3 protein determination. Finally, automated capillary Western assay's sensitivity and specificity were superior to those of WB assay (93.5 and 95.3%, respectively, compared to 92.2 and 84.8% for WB). In conclusion, with a shorter time of anal...Continue Reading

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Citations

May 24, 2018·International Journal of Molecular Sciences·Tatsuya KawaguchiMasafumi Matsuo
Dec 24, 2018·The Journal of Biological Chemistry·Andrew R CastleDavid Westaway
Mar 5, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Sophie EdouardMichel Drancourt

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Methods Mentioned

BETA
Protein
Assay
electrophoresis
ELISA

Software Mentioned

MedCalc®
Quantity One®
Compass for Simple Western®
Compass for Simple Western

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